Electrophoretic Deformation of Individual Transfer RNA Molecules Reveals Their Identity.

Autor:	Henley RY; Department of Physics, Northeastern University , Boston, Massachusetts 02115, United States., Ashcroft BA; Biodesign Institute, Arizona State University , Tempe, Arizona 85281, United States., Farrell I; Anima Cell Metrology, Inc. , Bernardsville, New Jersey 07924, United States., Cooperman BS; Department of Chemistry, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States., Lindsay SM; Department of Physics, Arizona State University , Tempe, Arizona 85281, United States.; Biodesign Institute, Arizona State University , Tempe, Arizona 85281, United States.; Department of Chemistry and Biochemistry, Arizona State University , Tempe, Arizona 85281, United States., Wanunu M; Department of Physics, Northeastern University , Boston, Massachusetts 02115, United States.; Department of Chemistry/Chemical Biology, Northeastern University , Boston, Massachusetts 02115, United States.
Jazyk:	angličtina
Zdroj:	Nano letters [Nano Lett] 2016 Jan 13; Vol. 16 (1), pp. 138-44. Date of Electronic Publication: 2015 Dec 02.
DOI:	10.1021/acs.nanolett.5b03331
Abstrakt:	It has been hypothesized that the ribosome gains additional fidelity during protein translation by probing structural differences in tRNA species. We measure the translocation kinetics of different tRNA species through ∼3 nm diameter synthetic nanopores. Each tRNA species varies in the time scale with which it is deformed from equilibrium, as in the translocation step of protein translation. Using machine-learning algorithms, we can differentiate among five tRNA species, analyze the ratios of tRNA binary mixtures, and distinguish tRNA isoacceptors.
Databáze:	MEDLINE
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