Fms-like tyrosine kinase 3 (Flt3) ligand depletes erythroid island macrophages and blocks medullar erythropoiesis in the mouse.

Autor: Jacobsen RN; Stem Cell Biology Group, Mater Research Institute, University of Queensland, Woolloongabba, Queensland, Australia; School of Medicine, University of Queensland, Herston, Queensland, Australia., Nowlan B; Stem Cell Biology Group, Mater Research Institute, University of Queensland, Woolloongabba, Queensland, Australia., Brunck ME; Stem Cell Biology Group, Mater Research Institute, University of Queensland, Woolloongabba, Queensland, Australia., Barbier V; Stem Cells and Cancer Group, Mater Research Institute, University of Queensland, Woolloongabba, Queensland, Australia., Winkler IG; Stem Cells and Cancer Group, Mater Research Institute, University of Queensland, Woolloongabba, Queensland, Australia., Levesque JP; Stem Cell Biology Group, Mater Research Institute, University of Queensland, Woolloongabba, Queensland, Australia; School of Medicine, University of Queensland, Herston, Queensland, Australia. Electronic address: jp.levesque@mater.uq.edu.au.
Jazyk: angličtina
Zdroj: Experimental hematology [Exp Hematol] 2016 Mar; Vol. 44 (3), pp. 207-12.e4. Date of Electronic Publication: 2015 Dec 01.
DOI: 10.1016/j.exphem.2015.11.004
Abstrakt: The cytokines granulocyte colony-stimulating factor (G-CSF) and Flt3 ligand (Flt3-L) mobilize hematopoietic stem and progenitor cells into the peripheral blood of primates, humans, and mice. We recently reported that G-CSF administration causes a transient blockade of medullar erythropoiesis by suppressing erythroblastic island (EI) macrophages in the bone marrow. In the study described here, we investigated the effect of mobilizing doses of Flt3-L on erythropoiesis in mice in vivo. Similar to G-CSF, Flt3-L caused whitening of the bone marrow with significant reduction in the numbers of EI macrophages and erythroblasts. This was compensated by an increase in the numbers of EI macrophages and erythroblasts in the spleen. However, unlike G-CSF, Flt3-L had an indirect effect on EI macrophages, as it was not detected at the surface of EI macrophages or erythroid progenitors.
(Copyright © 2016 ISEH - International Society for Experimental Hematology. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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