Super-Resolution Optical Fluctuation Bio-Imaging with Dual-Color Carbon Nanodots.

Autor: Chizhik AM; Third Institute of Physics, Georg August University , 37077 Göttingen, Germany., Stein S; Third Institute of Physics, Georg August University , 37077 Göttingen, Germany., Dekaliuk MO; A. V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine , Leontovicha Street 9, Kiev 01601, Ukraine., Battle C; Third Institute of Physics, Georg August University , 37077 Göttingen, Germany., Li W; Third Institute of Physics, Georg August University , 37077 Göttingen, Germany., Huss A; Third Institute of Physics, Georg August University , 37077 Göttingen, Germany., Platen M; Third Institute of Physics, Georg August University , 37077 Göttingen, Germany., Schaap IA; Third Institute of Physics, Georg August University , 37077 Göttingen, Germany.; Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot Watt University , Edinburgh EH14 4A, United Kingdom., Gregor I; Third Institute of Physics, Georg August University , 37077 Göttingen, Germany., Demchenko AP; A. V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine , Leontovicha Street 9, Kiev 01601, Ukraine., Schmidt CF; Third Institute of Physics, Georg August University , 37077 Göttingen, Germany., Enderlein J; Third Institute of Physics, Georg August University , 37077 Göttingen, Germany., Chizhik AI; Third Institute of Physics, Georg August University , 37077 Göttingen, Germany.
Jazyk: angličtina
Zdroj: Nano letters [Nano Lett] 2016 Jan 13; Vol. 16 (1), pp. 237-42. Date of Electronic Publication: 2015 Dec 02.
DOI: 10.1021/acs.nanolett.5b03609
Abstrakt: Success in super-resolution imaging relies on a proper choice of fluorescent probes. Here, we suggest novel easily produced and biocompatible nanoparticles-carbon nanodots-for super-resolution optical fluctuation bioimaging (SOFI). The particles revealed an intrinsic dual-color fluorescence, which corresponds to two subpopulations of particles of different electric charges. The neutral nanoparticles localize to cellular nuclei suggesting their potential use as an inexpensive, easily produced nucleus-specific label. The single particle study revealed that the carbon nanodots possess a unique hybrid combination of fluorescence properties exhibiting characteristics of both dye molecules and semiconductor nanocrystals. The results suggest that charge trapping and redistribution on the surface of the particles triggers their transitions between emissive and dark states. These findings open up new possibilities for the utilization of carbon nanodots in the various super-resolution microscopy methods based on stochastic optical switching.
Databáze: MEDLINE
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