Results of a Saxitoxin Proficiency Test Including Characterization of Reference Material and Stability Studies.

Autor:	Harju K; VERIFIN (Finnish Institute for Verification of the Chemical Weapons Convention),Department of Chemistry, P.O. Box 55, A. I. Virtasen aukio 1, University of Helsinki,FI-00014 Helsinki, Finland. kirsi.harju@helsinki.fi., Rapinoja ML; VERIFIN (Finnish Institute for Verification of the Chemical Weapons Convention),Department of Chemistry, P.O. Box 55, A. I. Virtasen aukio 1, University of Helsinki,FI-00014 Helsinki, Finland. marja-leena.rapinoja@helsinki.fi., Avondet MA; Federal Department of Defence, Civil Protection and Sport, SPIEZ LABORATORY, Austrasse 1,CH-3700 Spiez, Switzerland. marc-andre.avondet@babs.admin.ch., Arnold W; Federal Department of Defence, Civil Protection and Sport, SPIEZ LABORATORY, Austrasse 1,CH-3700 Spiez, Switzerland. werner.arnold@babs.admin.ch., Schär M; Federal Department of Defence, Civil Protection and Sport, SPIEZ LABORATORY, Austrasse 1,CH-3700 Spiez, Switzerland. martin.schaer@babs.admin.ch., Luginbühl W; ChemStat, Aarstrasse 98, CH-3005 Bern, Switzerland. info@chemstat.ch., Kremp A; Finnish Environment Institute, Marine Research Centre, Erik Palménin aukio 1,FI-00560 Helsinki, Finland. anke.kremp@ymparisto.fi., Suikkanen S; Finnish Environment Institute, Marine Research Centre, Erik Palménin aukio 1,FI-00560 Helsinki, Finland. sanna.suikkanen@ymparisto.fi., Kankaanpää H; Finnish Environment Institute, Marine Research Centre, Hakuninmaantie 6, FI-00430 Helsinki, Finland. harri.kankaanpaa@ymparisto.fi., Burrell S; Marine Institute, Marine Environment and Food Safety Services, Rinville, Oranmore,County Galway, Ireland. stephen.burrell@marine.ie., Söderström M; VERIFIN (Finnish Institute for Verification of the Chemical Weapons Convention),Department of Chemistry, P.O. Box 55, A. I. Virtasen aukio 1, University of Helsinki,FI-00014 Helsinki, Finland. martin.soderstrom@helsinki.fi., Vanninen P; VERIFIN (Finnish Institute for Verification of the Chemical Weapons Convention),Department of Chemistry, P.O. Box 55, A. I. Virtasen aukio 1, University of Helsinki,FI-00014 Helsinki, Finland. paula.vanninen@helsinki.fi.
Jazyk:	angličtina
Zdroj:	Toxins [Toxins (Basel)] 2015 Nov 25; Vol. 7 (12), pp. 4852-67. Date of Electronic Publication: 2015 Nov 25.
DOI:	10.3390/toxins7124852
Abstrakt:	A saxitoxin (STX) proficiency test (PT) was organized as part of the Establishment of Quality Assurance for the Detection of Biological Toxins of Potential Bioterrorism Risk (EQuATox) project. The aim of this PT was to provide an evaluation of existing methods and the European laboratories' capabilities for the analysis of STX and some of its analogues in real samples. Homogenized mussel material and algal cell materials containing paralytic shellfish poisoning (PSP) toxins were produced as reference sample matrices. The reference material was characterized using various analytical methods. Acidified algal extract samples at two concentration levels were prepared from a bulk culture of PSP toxins producing dinoflagellate Alexandrium ostenfeldii. The homogeneity and stability of the prepared PT samples were studied and found to be fit-for-purpose. Thereafter, eight STX PT samples were sent to ten participating laboratories from eight countries. The PT offered the participating laboratories the possibility to assess their performance regarding the qualitative and quantitative detection of PSP toxins. Various techniques such as official Association of Official Analytical Chemists (AOAC) methods, immunoassays, and liquid chromatography-mass spectrometry were used for sample analyses.
Databáze:	MEDLINE
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