Effects of Liposomal Compositions with Oxidized Dextrans on Functional Activity of U937 Macrophage-Like Cells In Vitro.

Autor: Kozhin PM; Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia., Chechushkov AV; Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia., Zaitseva NS; Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia., Lemza AE; Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia., Men'shchikova EB; Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia. lemen@centercem.ru., Troitskii AV; Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia., Shkurupy VA; Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia.; Novosibirsk State Medical University, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2015 Nov; Vol. 160 (1), pp. 57-60. Date of Electronic Publication: 2015 Nov 25.
DOI: 10.1007/s10517-015-3097-0
Abstrakt: We studied the effects of liposomal pharmaceutical compositions with oxidized dextrans on functional activity of U937 monocyte/macrophage-like cells. Liposomes in the emulsion contained oxidized dextran with a molecular weights of 40 kDa or 70 kDa or isonicotinic acid hydrazide (INAH) conjugated with oxidized dextran (40 kDa). Cell viability was evaluated by MTT test; mitochondrial transmembrane potential and production of superoxide anion and H2O2 were studied by fluorescent methods. The studied compositions exhibited no cytotoxic effect and even improved cell viability and mitochondrial respiration. Liposomes with oxidized 40 kDa dextran, including those with INAH-conjugated dextran, inhibited production of superoxide anion, but increased H2O2 generation.
Databáze: MEDLINE
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