Uptake of clinical yeast isolates by human epithelial cell line.

Autor: Atre AN; National Centre for Cell Science, Savitribai Phule Pune University Campus, Ganesh Khind, Pune 411007, India., Mehta A; National Centre for Cell Science, Savitribai Phule Pune University Campus, Ganesh Khind, Pune 411007, India., Chandorkar PR; National Centre for Cell Science, Savitribai Phule Pune University Campus, Ganesh Khind, Pune 411007, India., Patole MS; National Centre for Cell Science, Savitribai Phule Pune University Campus, Ganesh Khind, Pune 411007, India. Electronic address: patole@nccs.res.in., Diwanay SS; M.E.S. Abasaheb Garware College, Karve Road, Pune 411004, India., Shah SR; Bharati Vidyapeeth's Medical College, Pune 411043, India., Modak MS; Bharati Vidyapeeth's Medical College, Pune 411043, India.
Jazyk: angličtina
Zdroj: Journal de mycologie medicale [J Mycol Med] 2016 Sep; Vol. 26 (3), pp. 187-92. Date of Electronic Publication: 2015 Nov 17.
DOI: 10.1016/j.mycmed.2015.10.001
Abstrakt: Objective: The occurrence of yeast infections in humans has increased, with the species belonging to genus Candida still being the most common cause of infection. Nevertheless, infections caused by less common yeasts have been widely reported in recent years. The main objective of this study was to assess the potential of these less common saprophytic yeasts to invade the host cell, which is essential for causing systemic infections.
Material and Methods: Various yeast isolates were identified by DNA sequence information of PCR amplified ITS region. The purported saprophytic yeasts were characterized for internalization by mammalian cells in vitro, by staining the F-actin.
Conclusion: The identification of different yeast isolates from various patients revealed that 70% of the isolates belonged to the genus Candida, while remaining 30% of the isolates were yeasts not belonging to genus Candida. These non-Candida clinical isolates, either in yeast or hyphal forms, were efficiently internalized by human epithelial cells. The internalization was marked by a process of actin polymerization surrounding the invading yeast. Such uptake by epithelial cells signifies traversal of cell barrier by yeast cells during infection in vivo.
(Copyright © 2015 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: