| Autor: |
Beskrovnaia OIu, Bukanov NO, Okorokov AL, Fonshteĭn MIu, Iankovskiĭ NK |
| Jazyk: |
ruština |
| Zdroj: |
Genetika [Genetika] 1989 Jan; Vol. 25 (1), pp. 49-56. |
| Abstrakt: |
Molecular cloning and expression of Corynebacterium glutamicum genes complementing Escherichia coli mutations thrA2 and ilvA was performed. It was demonstrated that the thrA2 gene of C. glutamicum is located close to thrB on EcoRI DNA fragment 4.1 kb long. The fragment was cloned in pUC18 vector. The thrA2 gene is expressed in the recombinant plasmid pOBT3 under control of the vector pUC18 Plac promoter. In E. coli minicells, the genes thrA2 and thrB determined synthesis of proteins of Mr 43kD and 25 kD, respectively. A gene complementing ilvA mutation of E. coli was identified in a library of EcoRI C. glutamicum DNA fragments. This library was constructed using plasmid vector. It was shown that the ilvA gene of C. glutamicum is located inside the 3.6 kb EcoRI fragment and is expressed using its own promoter. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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