Periapical lesions decrease Akt serine phosphorylation and plasma membrane GLUT4 content in rat skeletal muscle.

Autor: Pereira RF; Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis, Department of Basic Sciences, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Rod. Marechal Rondom, km 527/528, Araçatuba, SP, 16018-805, Brazil., de Oliveira da Mota MS; Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis, Department of Basic Sciences, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Rod. Marechal Rondom, km 527/528, Araçatuba, SP, 16018-805, Brazil., de Lima Coutinho Mattera MS; Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis, Department of Basic Sciences, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Rod. Marechal Rondom, km 527/528, Araçatuba, SP, 16018-805, Brazil., Tsosura TV; Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis, Department of Basic Sciences, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Rod. Marechal Rondom, km 527/528, Araçatuba, SP, 16018-805, Brazil., Chiba FY; Department of Child and Social Dentistry, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Araçatuba, SP, Brazil., Garbin CA; Department of Child and Social Dentistry, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Araçatuba, SP, Brazil., Ervolino E; Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis, Department of Basic Sciences, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Rod. Marechal Rondom, km 527/528, Araçatuba, SP, 16018-805, Brazil., Cintra LT; Endodontics, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Araçatuba, SP, Brazil., Okamoto MM; Department of Physiology and Biophysics, São Paulo Institute of Biomedical Sciences, University of São Paulo (USP), Av. Prof. Lineu Prestes, 2415, CEP: 05508-900, São Paulo, Brazil., Machado UF; Department of Physiology and Biophysics, São Paulo Institute of Biomedical Sciences, University of São Paulo (USP), Av. Prof. Lineu Prestes, 2415, CEP: 05508-900, São Paulo, Brazil., Sumida DH; Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis, Department of Basic Sciences, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Rod. Marechal Rondom, km 527/528, Araçatuba, SP, 16018-805, Brazil. dorishs@foa.unesp.br.
Jazyk: angličtina
Zdroj: Clinical oral investigations [Clin Oral Investig] 2016 Sep; Vol. 20 (7), pp. 1625-30. Date of Electronic Publication: 2015 Nov 23.
DOI: 10.1007/s00784-015-1664-4
Abstrakt: Objectives: Periapical lesion (PL) promotes insulin resistance; however, the mechanisms underlying this alteration are not fully understood. Therefore, in this study, we aimed to evaluate the Akt serine phosphorylation status and GLUT4 expression levels in the gastrocnemius muscle (GM) of rats with PL.
Materials and Methods: Male Wistar rats (n = 42) were distributed equally into control (CN) and PL groups. The pulpal tissue of the PL group rats was exposed to the oral environment for 30 days. Thereafter, glucose and insulin levels were assessed, followed by homeostasis model assessment of insulin resistance (HOMA-IR). The Akt serine phosphorylation and GLUT4 levels of microsomal (M) and plasma membrane (PM) fractions were evaluated by western blotting and analyzed statistically.
Results: Compared to CN group rats, PL group rats had lower insulin sensitivity (as observed by HOMA-IR), lower Akt serine phosphorylation status after insulin stimulus, and lower GLUT4 levels in the PM fraction. However, the M fraction in the PL group did not differ significantly from that of the CN group.
Conclusions: PL decreases insulin sensitivity, Akt phosphorylation, and PM GLUT4 content.
Clinical Relevance: The present study indicates that preventing endodontic disease can thwart insulin resistance.
Databáze: MEDLINE
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