The dependence of chemokine-glycosaminoglycan interactions on chemokine oligomerization.

Autor:	Dyer DP; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, 9500 Gilman Drive MC0684, San Diego, La Jolla, CA 92093-0684, USA., Salanga CL; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, 9500 Gilman Drive MC0684, San Diego, La Jolla, CA 92093-0684, USA., Volkman BF; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, USA., Kawamura T; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, 9500 Gilman Drive MC0684, San Diego, La Jolla, CA 92093-0684, USA., Handel TM; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, 9500 Gilman Drive MC0684, San Diego, La Jolla, CA 92093-0684, USA thandel@ucsd.edu.
Jazyk:	angličtina
Zdroj:	Glycobiology [Glycobiology] 2016 Mar; Vol. 26 (3), pp. 312-26. Date of Electronic Publication: 2015 Nov 17.
DOI:	10.1093/glycob/cwv100
Abstrakt:	Both chemokine oligomerization and binding to glycosaminoglycans (GAGs) are required for their function in cell recruitment. Interactions with GAGs facilitate the formation of chemokine gradients, which provide directional cues for migrating cells. In contrast, chemokine oligomerization is thought to contribute to the affinity of GAG interactions by providing a more extensive binding surface than single subunits alone. However, the importance of chemokine oligomerization to GAG binding has not been extensively quantified. Additionally, the ability of chemokines to form different oligomers has been suggested to impart specificity to GAG interactions, but most studies have been limited to heparin. In this study, several differentially oligomerizing chemokines (CCL2, CCL3, CCL5, CCL7, CXCL4, CXCL8, CXCL11 and CXCL12) and select oligomerization-deficient mutants were systematically characterized by surface plasmon resonance to determine their relative affinities for heparin, heparan sulfate (HS) and chondroitin sulfate-A (CS-A). Wild-type chemokines demonstrated a hierarchy of binding affinities for heparin and HS that was markedly dependent on oligomerization. These results were corroborated by their relative propensity to accumulate on cells and the critical role of oligomerization in cell presentation. CS-A was found to exhibit greater chemokine selectivity than heparin or HS, as it only bound a subset of chemokines; moreover, binding to CS-A was ablated with oligomerization-deficient mutants. Overall, this study definitively demonstrates the importance of oligomerization for chemokine-GAG interactions, and demonstrates diversity in the affinity and specificity of different chemokines for GAGs. These data support the idea that GAG interactions provide a mechanism for fine-tuning chemokine function. (© The Author 2015. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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