HPLC determination of α-ketoglutaramate [5-amino-2,5-dioxopentanoate] in biological samples.

Autor: Shurubor YI; The Brain and Mind Research Institute, Weill Cornell Medical College, Department of Neurology, New York, NY 10021, USA; Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., Moscow 119071, Russia., Cooper AJ; Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA. Electronic address: arthur_cooper@nymc.edu., Isakova EP; Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., Moscow 119071, Russia., Deryabina YI; Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., Moscow 119071, Russia., Beal MF; The Brain and Mind Research Institute, Weill Cornell Medical College, Department of Neurology, New York, NY 10021, USA., Krasnikov BF; Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., Moscow 119071, Russia; Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA.
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2016 Feb 01; Vol. 494, pp. 52-4. Date of Electronic Publication: 2015 Nov 11.
DOI: 10.1016/j.ab.2015.11.003
Abstrakt: α-Ketoglutaramate is an important glutamine metabolite in mammals, plants, and many bacteria. It is also a nicotine metabolite in certain bacteria. Previously published methods for the determination of α-ketoglutaramate in biological samples have considerable drawbacks. Here, we describe a relatively simple high-performance liquid chromatography (HPLC)-based method for measurement of α-ketoglutaramate in plasma and deproteinized tissues that overcomes these drawbacks. Concentrations of α-ketoglutaramate in normal rat liver, kidney, brain, and plasma were found to be approximately 216, 13, 6, and 19 μM, respectively. The HPLC method should be useful for studying the role of α-ketoglutaramate in eukaryotic glutamine metabolism and in bacterial nicotine metabolism.
(Copyright © 2015 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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