The mystery of the fourth clone: comparative genomic analysis of four non-typeable Streptococcus pneumoniae strains with different susceptibilities to optochin.

Autor: Ikryannikova LN; Research Institute of Physical-Chemical Medicine, Ministry of Public Health of Russian Federation, 119992, Malaya Pirogovskaya str., 1a, Moscow, Russia. Larisa.Ikryannikova@gmail.com., Ischenko DS; Research Institute of Physical-Chemical Medicine, Ministry of Public Health of Russian Federation, 119992, Malaya Pirogovskaya str., 1a, Moscow, Russia., Lominadze GG; Federal State Budgetary Institute 'Scientific Center of Children Health' of RAMS, Moscow, Russia., Kanygina AV; Research Institute of Physical-Chemical Medicine, Ministry of Public Health of Russian Federation, 119992, Malaya Pirogovskaya str., 1a, Moscow, Russia., Karpova IY; Research Institute of Physical-Chemical Medicine, Ministry of Public Health of Russian Federation, 119992, Malaya Pirogovskaya str., 1a, Moscow, Russia., Kostryukova ES; Research Institute of Physical-Chemical Medicine, Ministry of Public Health of Russian Federation, 119992, Malaya Pirogovskaya str., 1a, Moscow, Russia., Mayansky NA; Federal State Budgetary Institute 'Scientific Center of Children Health' of RAMS, Moscow, Russia., Skvortsov VS; Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121, Russia., Ilina EN; Research Institute of Physical-Chemical Medicine, Ministry of Public Health of Russian Federation, 119992, Malaya Pirogovskaya str., 1a, Moscow, Russia., Govorun VM; Research Institute of Physical-Chemical Medicine, Ministry of Public Health of Russian Federation, 119992, Malaya Pirogovskaya str., 1a, Moscow, Russia.
Jazyk: angličtina
Zdroj: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2016 Jan; Vol. 35 (1), pp. 119-30. Date of Electronic Publication: 2015 Nov 12.
DOI: 10.1007/s10096-015-2516-5
Abstrakt: Optochin-resistant pneumococci can be rarely caught in clinical microbiology laboratories because of the routine identification of all such strains as viridans group non-pneumococci. We were lucky to find four non-typeable Streptococcus pneumoniae clones demonstrating the different susceptibilities to optochin: one of them (Spn_13856) was resistant to optochin, while the other three (Spn_1719, Spn_27, and Spn_2298) were susceptible. Whole genome nucleotide sequences of these strains were compared to reveal the differences between the optochin-resistant and optochin-susceptible strains. Two adjacent genes coding maltose O-acetyltransferase and uridine phosphorylase which were presented in the genomes of all optochin-susceptible strains and missed in the optochin-resistant strain were revealed. Non-synonymous substitutions in 14 protein-coding genes were discovered, including the Ala49Ser mutation in the C-subunit of the F0 part of the ATP synthase rotor usually associated with pneumococcal optochin resistance. Modeling of a process of optochin interaction with the F0 part of the ATP synthase rotor indicates that the complex of optochin with "domain C" composed by wild-type C-subunits is more stable than the same complex composed of Ala49Ser mutant C-subunits.
Databáze: MEDLINE
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