4D microvascular imaging based on ultrafast Doppler tomography.

Autor: Demené C; Institut Langevin, ESPCI ParisTech, Paris Sorbonne Lettres Research University, CNRS UMR7587, INSERM U979, Paris, France. Electronic address: charlie.demene@gmail.com., Tiran E; Institut Langevin, ESPCI ParisTech, Paris Sorbonne Lettres Research University, CNRS UMR7587, INSERM U979, Paris, France., Sieu LA; Institut de Biologie Paris Seine, INSERM U1130, CNRS UMR8246, University Pierre & Marie Curie UMCR18, Paris, France., Bergel A; Institut de Biologie Paris Seine, INSERM U1130, CNRS UMR8246, University Pierre & Marie Curie UMCR18, Paris, France., Gennisson JL; Institut Langevin, ESPCI ParisTech, Paris Sorbonne Lettres Research University, CNRS UMR7587, INSERM U979, Paris, France., Pernot M; Institut Langevin, ESPCI ParisTech, Paris Sorbonne Lettres Research University, CNRS UMR7587, INSERM U979, Paris, France., Deffieux T; Institut Langevin, ESPCI ParisTech, Paris Sorbonne Lettres Research University, CNRS UMR7587, INSERM U979, Paris, France., Cohen I; Institut de Biologie Paris Seine, INSERM U1130, CNRS UMR8246, University Pierre & Marie Curie UMCR18, Paris, France., Tanter M; Institut Langevin, ESPCI ParisTech, Paris Sorbonne Lettres Research University, CNRS UMR7587, INSERM U979, Paris, France.
Jazyk: angličtina
Zdroj: NeuroImage [Neuroimage] 2016 Feb 15; Vol. 127, pp. 472-483. Date of Electronic Publication: 2015 Nov 10.
DOI: 10.1016/j.neuroimage.2015.11.014
Abstrakt: 4D ultrasound microvascular imaging was demonstrated by applying ultrafast Doppler tomography (UFD-T) to the imaging of brain hemodynamics in rodents. In vivo real-time imaging of the rat brain was performed using ultrasonic plane wave transmissions at very high frame rates (18,000 frames per second). Such ultrafast frame rates allow for highly sensitive and wide-field-of-view 2D Doppler imaging of blood vessels far beyond conventional ultrasonography. Voxel anisotropy (100 μm × 100 μm × 500 μm) was corrected for by using a tomographic approach, which consisted of ultrafast acquisitions repeated for different imaging plane orientations over multiple cardiac cycles. UFT-D allows for 4D dynamic microvascular imaging of deep-seated vasculature (up to 20 mm) with a very high 4D resolution (respectively 100 μm × 100 μm × 100 μm and 10 ms) and high sensitivity to flow in small vessels (>1 mm/s) for a whole-brain imaging technique without requiring any contrast agent. 4D ultrasound microvascular imaging in vivo could become a valuable tool for the study of brain hemodynamics, such as cerebral flow autoregulation or vascular remodeling after ischemic stroke recovery, and, more generally, tumor vasculature response to therapeutic treatment.
(Copyright © 2015 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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