Proteomic analysis of hyperadhesive Candida glabrata clinical isolates reveals a core wall proteome and differential incorporation of adhesins.

Autor:	Gómez-Molero E; Regional Center for Biomedical Research, Albacete Science & Technology Park, University of Castilla-La Mancha, E-02008 Albacete, Spain Institute for Medical Microbiology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany., de Boer AD; Regional Center for Biomedical Research, Albacete Science & Technology Park, University of Castilla-La Mancha, E-02008 Albacete, Spain., Dekker HL; Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, NL-1098 XH Amsterdam, the Netherlands., Moreno-Martínez A; Regional Center for Biomedical Research, Albacete Science & Technology Park, University of Castilla-La Mancha, E-02008 Albacete, Spain., Kraneveld EA; Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, NL-1081 LA Amsterdam, the Netherlands., Ichsan; Institute for Medical Microbiology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany., Chauhan N; Public Health Research Institute and Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers University, 225 Warren Street, Newark, NJ 07103, USA., Weig M; Institute for Medical Microbiology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany., de Soet JJ; Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, NL-1081 LA Amsterdam, the Netherlands., de Koster CG; Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, NL-1098 XH Amsterdam, the Netherlands., Bader O; Institute for Medical Microbiology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany., de Groot PW; Regional Center for Biomedical Research, Albacete Science & Technology Park, University of Castilla-La Mancha, E-02008 Albacete, Spain piet.degroot@uclm.es.
Jazyk:	angličtina
Zdroj:	FEMS yeast research [FEMS Yeast Res] 2015 Dec; Vol. 15 (8). Date of Electronic Publication: 2015 Nov 05.
DOI:	10.1093/femsyr/fov098
Abstrakt:	Attachment to human host tissues or abiotic medical devices is a key step in the development of infections by Candida glabrata. The genome of this pathogenic yeast codes for a large number of adhesins, but proteomic work using reference strains has shown incorporation of only few adhesins in the cell wall. By making inventories of the wall proteomes of hyperadhesive clinical isolates and reference strain CBS138 using mass spectrometry, we describe the cell wall proteome of C. glabrata and tested the hypothesis that hyperadhesive isolates display differential incorporation of adhesins. Two clinical strains (PEU382 and PEU427) were selected, which both were hyperadhesive to polystyrene and showed high surface hydrophobicity. Cell wall proteome analysis under biofilm-forming conditions identified a core proteome of about 20 proteins present in all C. glabrata strains. In addition, 12 adhesin-like wall proteins were identified in the hyperadherent strains, including six novel adhesins (Awp8-13) of which only Awp12 was also present in CBS138. We conclude that the hyperadhesive capacity of these two clinical C. glabrata isolates is correlated with increased and differential incorporation of cell wall adhesins. Future studies should elucidate the role of the identified proteins in the establishment of C. glabrata infections. (© FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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