Cytotoxic Activities of Eosinophil Cationic Protein and Eosinophil-derived Neurotoxin: In Silico Analysis.
| Autor: | De Oliveira PC; Laboratory of Molecular Biology, Federal University of São João Del Rei-Midwest Campus Dona Lindu, Divinópolis, Brazil., De Oliveira Lopes D; Laboratory of Molecular Biology, Federal University of São João Del Rei-Midwest Campus Dona Lindu, Divinópolis, Brazil., Do Vale Coelho IE; Laboratory of Molecular Modeling, Federal University of São João Del Rei-Midwest Campus Dona Lindu, Divinópolis, Brazil., Pereira MC; Laboratory of Molecular Biology, Federal University of São João Del Rei-Midwest Campus Dona Lindu, Divinópolis, Brazil michelepereira@ufsj.edu.br. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Cancer genomics & proteomics [Cancer Genomics Proteomics] 2015 Nov-Dec; Vol. 12 (6), pp. 397-402. |
| Abstrakt: | Background: Eosinophil cationic protein (ECP) and eosinophil derived-neurotoxin (EDN) are homologous ribonuclease (RNAse) A family proteins. The objective of the present study was to in silico characterize ECP and EDN with respect to their cytotoxic activities. Materials and Methods: Structural, physicochemical, and conserved domain characterizations were carried-out using open-source software, such as InterProScan, NetOGlyc, NetPhos and Discovery Studio 3.1. Results: The proteins did not have atypical conserved domains. EDN had a greater number of glutamine amino acid residues, whereas ECP had a predominance of arginine. ECP had four possible N-glycosylation, three O-glycosylation and four phosphorylation sites. EDN had five putative N-glycosylation, three phosphorylation and no O-glycosylation sites. Conclusion: The greater cationicity of ECP may be related to its higher cytotoxicity and to the fact that the varying post-translational modification profiles can generate functional differences from structural alteration. In vivo and in vitro studies need to be performed in order to confirm these predictions. (Copyright© 2015, International Institute of Anticancer Research (Dr. John G. Delinasios), All rights reserved.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|