Cryptochrome-dependent circadian periods in the arcuate nucleus.

Autor: Uchida H; Laboratory of Oral Chronobiology, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan; Department of Oral-Facial Disorders, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan., Nakamura TJ; Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, Kanagawa 214-8571, Japan; Faculty of Pharmaceutical Sciences, Teikyo Heisei University, Tokyo 164-8530, Japan., Takasu NN; Laboratory of Oral Chronobiology, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan., Todo T; Department of Radiation Biology and Medical Genetics, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan., Sakai T; Department of Oral-Facial Disorders, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan., Nakamura W; Laboratory of Oral Chronobiology, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan. Electronic address: wataru@dent.osaka-u.ac.jp.
Jazyk: angličtina
Zdroj: Neuroscience letters [Neurosci Lett] 2016 Jan 01; Vol. 610, pp. 123-8. Date of Electronic Publication: 2015 Nov 02.
DOI: 10.1016/j.neulet.2015.10.071
Abstrakt: The circadian pacemaker in the suprachiasmatic nucleus (SCN) of the hypothalamus is responsible for controlling behavioral activity rhythms, such as a free running rhythm in constant darkness. Rodents have several circadian oscillators in other brain regions including the arcuate nucleus (ARC). In specific conditions such as food anticipatory activity rhythms in the context of timed restricted feeding, an alternative circadian pace-making system has been assumed by means of circadian oscillators like the SCN. Despite extensive lesion studies, the anatomic locations of extra-SCN circadian pacemakers responsible for regulating behavioral rhythms have not been found. In the present study, we investigated circadian rhythms in the SCN and extra-SCN region of the arcuate nucleus (ARC) by analyzing PER2::LUCIFERASE expression in specific regions from wild-type C57BL/6, Cry1(-/-), and Cry2(-/-) mice. Compared to wild-type animals, we observed period shortening in both the SCN and ARC of Cry1(-/-) mice and period lengthening in Cry2(-/-) mice. Interestingly, the periods in the ARC of both genotypes were identical to those in the SCN. Moreover, the amplitudes of PER2::LUC rhythms in the ARC of all animals were decreased compared to those in the SCN. These data suggest that the ARC is a candidate circadian pacemaker outside the SCN.
(Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: