A Microfluidic Device for Immunoassay-Based Protein Analysis of Single E. coli Bacteria.

Autor: Stratz S; Department of Biosystems Science and Engineering, ETH Zurich, Vladimir-Prelog-Weg 3, Zurich, 8091, Switzerland., Dittrich PS; Department of Biosystems Science and Engineering, ETH Zurich, Vladimir-Prelog-Weg 3, Zurich, 8091, Switzerland. petra.dittrich@bsse.ethz.ch.
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2015; Vol. 1346, pp. 11-25.
DOI: 10.1007/978-1-4939-2987-0_2
Abstrakt: We present a method suitable for quantitative analysis of intracellular proteins, metabolites and secondary messengers of single bacterial cells. The method integrates the concept of immunoassays on a microfluidic device that facilitates single cell trapping and isolating in a small volume of a few tens of picoliters. Combination of the benefits of microfluidic systems for single cell analysis with the high analytical selectivity and sensitivity of immunoassays enables the detection of even low abundant intracellular analytes which occur only at a few hundred copies per bacterium.
Databáze: MEDLINE