Evaluation of affinity interaction between small molecules and platelets by open tubular affinity capillary electrochromatography.

Autor: Wang FQ; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China., Zhang Q; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China., Li CH; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China., Wang YZ; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China., Hu YJ; State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, P. R. China., Zhang QH; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China., Xia ZN; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China., Yang FQ; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China.
Jazyk: angličtina
Zdroj: Electrophoresis [Electrophoresis] 2016 Mar; Vol. 37 (5-6), pp. 736-43. Date of Electronic Publication: 2015 Dec 09.
DOI: 10.1002/elps.201500414
Abstrakt: In this paper, an open tubular affinity capillary electrochromatography (OT-ACEC) was developed by physical adsorption of rabbit platelets on the inner surface of capillary. The interactions between small molecules include adenosine diphosphate (ADP) (positive control), protocatechuic acid (negative control) and seven natural products (salvianolic acid B, salvianic acid A sodium, hydroxysafflor yellow A, ferulic acid, chlorogenic acid, sinapic acid, caffeic acid) and platelets were evaluated by their retention factors and binding constants obtained based on peak-shift assay. Then, the activities of anti-platelet aggregation induced by thrombin (THR), ADP and arachidonic acid (AA) for those small molecules (except ADP) were evaluated by turbidimetric method. The results indicate that: (i) ADP, a platelet aggregation inducer, had strong interaction with platelet, while protocatechuic acid that had no inhibition on platelet aggregation behaved no specific interaction; (ii) there was a positive correlation between the anti-platelet aggregation activities of small molecules and their interactions with platelet, generally those compounds with higher binding constants with platelet exhibited higher activities. Therefore, the OT-ACEC method developed in the present study can be a potential method to evaluate affinity interactions between small molecules and platelets, so as to predict the biological activities such as anti-platelet aggregation for the small molecules.
(© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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