Controlling transcription in human pluripotent stem cells using CRISPR-effectors.

Autor: Genga RM; Program in Molecular Medicine, Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, MA 01605, USA., Kearns NA; Program in Molecular Medicine, Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, MA 01605, USA., Maehr R; Program in Molecular Medicine, Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, MA 01605, USA. Electronic address: rene.maehr@umassmed.edu.
Jazyk: angličtina
Zdroj: Methods (San Diego, Calif.) [Methods] 2016 May 15; Vol. 101, pp. 36-42. Date of Electronic Publication: 2015 Oct 23.
DOI: 10.1016/j.ymeth.2015.10.014
Abstrakt: The ability to manipulate transcription in human pluripotent stem cells (hPSCs) is fundamental for the discovery of key genes and mechanisms governing cellular state and differentiation. Recently developed CRISPR-effector systems provide a systematic approach to rapidly test gene function in mammalian cells, including hPSCs. In this review, we discuss recent advances in CRISPR-effector technologies that have been employed to control transcription through gene activation, gene repression, and epigenome engineering. We describe an application of CRISPR-effector mediated transcriptional regulation in hPSCs by targeting a synthetic promoter driving a GFP transgene, demonstrating the ease and effectiveness of CRISPR-effector mediated transcriptional regulation in hPSCs.
(Copyright © 2015 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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