Mechanistic modeling of enzymatic hydrolysis of cellulose integrating substrate morphology and cocktail composition.

Autor: Huron M; IFP Energies Nouvelles, Rond-point de l'échangeur de Solaize - BP 3, 69360 Solaize, France., Hudebine D; IFP Energies Nouvelles, Rond-point de l'échangeur de Solaize - BP 3, 69360 Solaize, France. damien.hudebine@ifpen.fr., Lopes Ferreira N; IFP Energies Nouvelles, 1 & 4 Avenue de Bois-Préau, Rueil-Malmaison Cedex, France., Lachenal D; Grenoble INP-Pagora, 461 rue de la Papeterie BP65, Saint Martin d'Hères Cedex, France.
Jazyk: angličtina
Zdroj: Biotechnology and bioengineering [Biotechnol Bioeng] 2016 May; Vol. 113 (5), pp. 1011-23. Date of Electronic Publication: 2015 Nov 26.
DOI: 10.1002/bit.25873
Abstrakt: A mechanistic model of enzymatic hydrolysis taking into account the morphology of the cellulosic particles and its evolution with time was developed. The individual behavior of the main enzymes involved in the reaction (cellobiohydrolases, endoglucanases, and β-glucosidases), as well as synergy effects, were also included. A large panel of experimental tests was done to fit and validate the model. This database included different enzymes mixtures and operating conditions and allowed to determine and compare with accuracy the adsorption and kinetic parameters of the different enzymes. Model predictions on short hydrolysis times were very satisfactory. On longer times, a deactivation constant was added to represent the hydrolysis slowdown. The model also allowed to predict the impact of enzymes ratios and initial substrate parameters (chain length distribution, polymerization degree) on hydrolysis, and to follow the evolution of these parameters with time. This model revealed general trends on the impact of cellulose morphology on hydrolysis. It is a useful tool to better understand the mechanisms involved in enzymatic hydrolysis of cellulose and to determine optimal cellulolytic cocktails for process design.
(© 2015 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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