Hydroalcoholic extract of red propolis promotes functional recovery and axon repair after sciatic nerve injury in rats.

Autor: Barbosa RA; a Department of Healthy and Environment, Tiradentes University , Aracaju , Brazil., Nunes TL; a Department of Healthy and Environment, Tiradentes University , Aracaju , Brazil., Nunes TL; a Department of Healthy and Environment, Tiradentes University , Aracaju , Brazil., da Paixão AO; a Department of Healthy and Environment, Tiradentes University , Aracaju , Brazil., Belo Neto R; a Department of Healthy and Environment, Tiradentes University , Aracaju , Brazil., Moura S; b Department of Technology, University of Caxias Do Sul , Caxias Do Sul , Brazil., Albuquerque Junior RL; a Department of Healthy and Environment, Tiradentes University , Aracaju , Brazil.; c Department of Biomaterials, Morphology and Experimental Pathology, Research and Technology Institute (ITP) , Aracaju , Brazil., Cândido EA; a Department of Healthy and Environment, Tiradentes University , Aracaju , Brazil.; c Department of Biomaterials, Morphology and Experimental Pathology, Research and Technology Institute (ITP) , Aracaju , Brazil., Padilha FF; a Department of Healthy and Environment, Tiradentes University , Aracaju , Brazil.; c Department of Biomaterials, Morphology and Experimental Pathology, Research and Technology Institute (ITP) , Aracaju , Brazil., Quintans-Júnior LJ; d Department of Physiology , Federal University of Sergipe , São Cristóvão , Brazil., Gomes MZ; a Department of Healthy and Environment, Tiradentes University , Aracaju , Brazil.; c Department of Biomaterials, Morphology and Experimental Pathology, Research and Technology Institute (ITP) , Aracaju , Brazil., Cardoso JC; a Department of Healthy and Environment, Tiradentes University , Aracaju , Brazil.; c Department of Biomaterials, Morphology and Experimental Pathology, Research and Technology Institute (ITP) , Aracaju , Brazil.
Jazyk: angličtina
Zdroj: Pharmaceutical biology [Pharm Biol] 2016; Vol. 54 (6), pp. 993-1004. Date of Electronic Publication: 2015 Oct 29.
DOI: 10.3109/13880209.2015.1091844
Abstrakt: Context: Peripheral axon injury and degeneration are often mediated by oxidative stress and inflammation. The hydroalcoholic extract of the red propolis (HERP) has attracted great attention because of its antioxidant and anti-inflammatory activities.
Objective: The objective of this work is to study the effect of HERP on nerve repair and functional recovery after sciatic nerve injury (SNI) in rats.
Materials and Methods: The chemical markers in HERP were identified using high-resolution mass spectroscopy. After axonotmesis of sciatic nerve, ibuprofen (IBP) and HERP treatments were orally administered for 28 d. Behavioural tests were performed weekly after SNI. The myelinated axon number was counted using morphometric analysis.
Results: The compounds found in HERP were pinocembrin, formononetin, vestitol, and biochanin A. The animals that underwent SNI showed a significant decrease in motor function based on the Basso, Beattie and Bresnahan scale and sciatic functional index compared with sham animals until 7 d after the surgery (p < 0.05). After 14 and 21 d, the SNI groups treated with either HERP or IBP showed significant improvement (p < 0.01), and the SNI group treated with HERP 10 mg/kg showed accelerated motor recovery compared with the other groups (p < 0.01). SNI caused also a reduction in the myelinated axon counts, and treatment with HERP 10 mg/kg induced a significant increase in the number of myelinated fibres compared with all other groups.
Conclusion: HERP promoted regenerative responses and accelerated functional recovery after sciatic nerve crush. Thus, it can be considered to be a new strategy or complementary therapy for treating nerve injuries.
Databáze: MEDLINE
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