Reduction of MDHAR activity in cherry tomato suppresses growth and yield and MDHAR activity is correlated with sugar levels under high light.

Autor: Truffault V; INRA, UR-1052, Génétique et Amélioration des Fruits et Légumes, CS60094, 84143, Montfavet, France.; INRA, UR-1115, Plantes et Systèmes de Culture Horticoles, CS40509, 84914, Avignon Cedex 9, France., Gest N; INRA, UR-1052, Génétique et Amélioration des Fruits et Légumes, CS60094, 84143, Montfavet, France., Garchery C; INRA, UR-1052, Génétique et Amélioration des Fruits et Légumes, CS60094, 84143, Montfavet, France., Florian A; Max-Planck-Institute of Molecular Plant Physiology, Am Muehlenberf 1, 14476, Potsdam-Golm, Germany., Fernie AR; Max-Planck-Institute of Molecular Plant Physiology, Am Muehlenberf 1, 14476, Potsdam-Golm, Germany., Gautier H; INRA, UR-1115, Plantes et Systèmes de Culture Horticoles, CS40509, 84914, Avignon Cedex 9, France., Stevens RG; INRA, UR-1052, Génétique et Amélioration des Fruits et Légumes, CS60094, 84143, Montfavet, France.
Jazyk: angličtina
Zdroj: Plant, cell & environment [Plant Cell Environ] 2016 Jun; Vol. 39 (6), pp. 1279-92. Date of Electronic Publication: 2016 Feb 10.
DOI: 10.1111/pce.12663
Abstrakt: Ascorbate is oxidized into the radical monodehydroascorbate (MDHA) through ascorbate oxidase or peroxidase activity or non-enzymatically by reactive oxygen species. Regeneration of ascorbate from MDHA is ensured by the enzyme MDHA reductase (MDHAR). Previous work has shown that growth processes and yield can be altered by modifying the activity of enzymes that recycle ascorbate; therefore, we have studied similar processes in cherry tomato (Solanum lycopersium L.) under- or overexpressing MDHAR. Physiological and metabolic characterization of these lines was carried out under different light conditions or by manipulating the source-sink ratio. Independently of the light regime, slower early growth of all organs was observed in MDHAR silenced lines, decreasing final fruit yield. Photosynthesis was altered as was the accumulation of hexoses and sucrose in a light-dependent manner in plantlets. Sucrose accumulation was also repressed in young fruits and final yield of MDHAR silenced lines showed a stronger decrease under carbon limitation, and the phenotype was partially restored by reducing fruit load. Ascorbate and MDHA appear to be involved in control of growth and sugar metabolism in cherry tomato and the associated enzymes could be potential targets for yield improvement.
(© 2016 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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