| Autor: |
Castel G; INRA-UMR 1062 CBGP, 755 Avenue Campus Agropolis, CS30016, 34988 Montferrier sur Lez, France. guillaume.castel@supagro.inra.fr.; Institut de Biologie Computationnelle, 34095 Montpellier, France. guillaume.castel@supagro.inra.fr., Couteaudier M; INRA-UR1282, Biologie Virus Aviaire, 37380 Nouzilly, France. mathilde.couteaudier@hotmail.fr., Sauvage F; CNRS-Université Lyon 1, Laboratoire de Biométrie et Biologie Evolutive (UMR5558), F-69622 Villeurbanne, France. sauvage_frank@yahoo.fr.; LabEx ECOFECT Ecoevolutionary Dynamics of Infectious Diseases, 69622 Villeurbanne, France. sauvage_frank@yahoo.fr., Pons JB; CNRS-Université Lyon 1, Laboratoire de Biométrie et Biologie Evolutive (UMR5558), F-69622 Villeurbanne, France. jean-baptiste.pons@universite-lyon.fr.; LabEx ECOFECT Ecoevolutionary Dynamics of Infectious Diseases, 69622 Villeurbanne, France. jean-baptiste.pons@universite-lyon.fr., Murri S; ANSES-Laboratoire de Lyon, Unité Virologie, 31 Avenue Tony Garnier, 69007 Lyon, France. severine.murri@anses.fr., Plyusnina A; Department of Virology, University of Helsinki, Helsinki FI-00014, Finland. angelina.plyusnina@helsinki.fi., Pontier D; CNRS-Université Lyon 1, Laboratoire de Biométrie et Biologie Evolutive (UMR5558), F-69622 Villeurbanne, France. dominique.pontier@univ-lyon1.fr.; LabEx ECOFECT Ecoevolutionary Dynamics of Infectious Diseases, 69622 Villeurbanne, France. dominique.pontier@univ-lyon1.fr., Cosson JF; INRA-UMR 1062 CBGP, 755 Avenue Campus Agropolis, CS30016, 34988 Montferrier sur Lez, France. cosson@supagro.inra.fr.; INRA-UMR Bipar, 23 Av. Général de Gaulle, 94706 Maisons-Alfort, France. cosson@supagro.inra.fr., Plyusnin A; Department of Virology, University of Helsinki, Helsinki FI-00014, Finland. alexander.plyusnin@helsinki.fi., Marianneau P; ANSES-Laboratoire de Lyon, Unité Virologie, 31 Avenue Tony Garnier, 69007 Lyon, France. philippe.marianneau@anses.fr., Tordo N; Institut Pasteur, Unité des Stratégies Antivirales, WHO collaborative Centre for Viral Haemorrhagic Fevers and Arboviruses, 25 rue du Docteur Roux, 75015 Paris, France. noel.tordo@pasteur.fr. |
| Abstrakt: |
Puumala virus (PUUV) is the agent of nephropathia epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS) in Europe. NE incidence presents a high spatial variation throughout France, while the geographical distribution of the wild reservoir of PUUV, the bank vole, is rather continuous. A missing piece of the puzzle is the current distribution and the genetic variation of PUUV in France, which has been overlooked until now and remains poorly understood. During a population survey, from 2008 to 2011, bank voles were trapped in eight different forests of France located in areas known to be endemic for NE or in area from where no NE case has been reported until now. Bank voles were tested for immunoglobulin (Ig)G ELISA serology and two seropositive animals for each of three different areas (Ardennes, Jura and Orleans) were then subjected to laboratory analyses in order to sequence the whole S, M and L segments of PUUV. Phylogenetic analyses revealed that French PUUV isolates globally belong to the central European (CE) lineage although isolates from Ardennes are clearly distinct from those in Jura and Orleans, suggesting a different evolutionary history and origin of PUUV introduction in France. Sequence analyses revealed specific amino acid signatures along the N protein, including in PUUV from the Orleans region from where NE in humans has never been reported. The relevance of these mutations in term of pathophysiology is discussed. |
