| Autor: |
Wahl EA; Department of Plastic Surgery and Hand Surgery, University Hospital Klinikum rechts der Isar, Technical University Munich, 81675 Munich, Germany., Fierro FA; Institute for Regenerative Cures, UC Davis, Sacramento, CA 95817, USA., Peavy TR; Department of Biological Sciences, California State University, Sacramento, CA 95819, USA., Hopfner U; Department of Plastic Surgery and Hand Surgery, University Hospital Klinikum rechts der Isar, Technical University Munich, 81675 Munich, Germany., Dye JF; Institute of Biomedical Engineering, UCL, The Royal Institution, London W1S 4BS, UK., Machens HG; Department of Plastic Surgery and Hand Surgery, University Hospital Klinikum rechts der Isar, Technical University Munich, 81675 Munich, Germany., Egaña JT; Department of Plastic Surgery and Hand Surgery, University Hospital Klinikum rechts der Isar, Technical University Munich, 81675 Munich, Germany ; FONDAP Center for Genome Regulation, Faculty of Sciences, University of Chile, 7800024 Santiago, Chile., Schenck TL; Department of Plastic Surgery and Hand Surgery, University Hospital Klinikum rechts der Isar, Technical University Munich, 81675 Munich, Germany. |
| Abstrakt: |
Mesenchymal stem cells (MSCs) have been shown to improve tissue regeneration in several preclinical and clinical trials. These cells have been used in combination with three-dimensional scaffolds as a promising approach in the field of regenerative medicine. We compare the behavior of human adipose-derived MSCs (AdMSCs) on four different biomaterials that are awaiting or have already received FDA approval to determine a suitable regenerative scaffold for delivering these cells to dermal wounds and increasing healing potential. AdMSCs were isolated, characterized, and seeded onto scaffolds based on chitosan, fibrin, bovine collagen, and decellularized porcine dermis. In vitro results demonstrated that the scaffolds strongly influence key parameters, such as seeding efficiency, cellular distribution, attachment, survival, metabolic activity, and paracrine release. Chick chorioallantoic membrane assays revealed that the scaffold composition similarly influences the angiogenic potential of AdMSCs in vivo. The wound healing potential of scaffolds increases by means of a synergistic relationship between AdMSCs and biomaterial resulting in the release of proangiogenic and cytokine factors, which is currently lacking when a scaffold alone is utilized. Furthermore, the methods used herein can be utilized to test other scaffold materials to increase their wound healing potential with AdMSCs. |
