Electrochemotherapy - Evidence for Cell-type Selectivity In Vitro.

Autor:	Landström F; Department of Otolaryngology, Örebro University Hospital, Örebro, Sweden School of Health and Medical Sciences, Örebro University, Örebro, Sweden fredrik.landstrom@orebroll.se., Ivarsson M; School of Health and Medical Sciences, Örebro University, Örebro, Sweden., Von Sydow AK; Clinical Research Center, Örebro University Hospital, Örebro, Sweden., Magnuson A; School of Health and Medical Sciences, Örebro University, Örebro, Sweden Clinical Research Center, Örebro University Hospital, Örebro, Sweden., Von Beckerath M; Department of Otolaryngology, Örebro University Hospital, Örebro, Sweden School of Health and Medical Sciences, Örebro University, Örebro, Sweden., Möller C; School of Health and Medical Sciences, Örebro University, Örebro, Sweden.
Jazyk:	angličtina
Zdroj:	Anticancer research [Anticancer Res] 2015 Nov; Vol. 35 (11), pp. 5813-20.
Abstrakt:	Aim: Electrochemotherapy (ECT) is a new cancer treatment modality that uses electroporation to potentiate chemotherapeutic agents, especially bleomycin. ECT causes both a direct toxic effect and an anti-vascular effect. The aim of the present study was to investigate a possible selective effect of ECT on the survival of fibroblasts, endothelial cells (HUVEC) and two squamous cell carcinoma cell lines (CAL-27 and SCC-4). Materials and Methods: Cells were electroporated using two bleomycin concentrations. The survival rate was assessed 1, 2, 3 and 4 days after treatment, by two different assays. Results: The survival rate of the fibroblasts was statistically significantly higher than the other cell lines at day 4. The HUVEC survival rate was statistically significantly lower than the other cell types at day 1 after electroporation-alone. Conclusion: A selective survival effect after ECT was observed in vitro, supporting the anti-vascular effect seen in vivo. (Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.)
Databáze:	MEDLINE
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