miR-19a and SOCS-1 expression in the differential diagnosis of laryngeal (glottic) verrucous squamous cell carcinoma.

Autor:	Marioni G; Department of Neurosciences DNS, Otolaryngology Section, Padova University, Padova, Italy., Agostini M; Department of Surgical, Oncological and Gastroenterological Sciences, Padova University, Padova, Italy Istituto di Ricerca Pediatrica-Città della Speranza, Padova, Italy Nanomedicine Department, The Methodist Hospital Research Institute, Houston, Texas, USA., Cappellesso R; Department of Medicine DIMED, University of Padova, Padova, Italy., Bedin C; Department of Surgical, Oncological and Gastroenterological Sciences, Padova University, Padova, Italy Istituto di Ricerca Pediatrica-Città della Speranza, Padova, Italy., Ottaviano G; Department of Neurosciences DNS, Otolaryngology Section, Padova University, Padova, Italy., Marchese-Ragona R; Department of Neurosciences DNS, Otolaryngology Section, Padova University, Padova, Italy., Lovato A; Department of Neurosciences DNS, Otolaryngology Section, Padova University, Padova, Italy., Cacco T; Department of Neurosciences DNS, Otolaryngology Section, Padova University, Padova, Italy., Giacomelli L; Department of Medicine DIMED, University of Padova, Padova, Italy., Nitti D; Department of Surgical, Oncological and Gastroenterological Sciences, Padova University, Padova, Italy., Blandamura S; Department of Medicine DIMED, University of Padova, Padova, Italy., Stellini E; Department of Neurosciences DNS, Odontostomatology Institute, Padova University, Padova, Italy., de Filippis C; Department of Neurosciences DNS, Audiology Unit, Treviso Hospital, Padova University, Treviso, Italy.
Jazyk:	angličtina
Zdroj:	Journal of clinical pathology [J Clin Pathol] 2016 May; Vol. 69 (5), pp. 415-21. Date of Electronic Publication: 2015 Oct 26.
DOI:	10.1136/jclinpath-2015-203308
Abstrakt:	Background: Laryngeal verrucous squamous cell carcinoma (VSCC) is a highly differentiated squamous cell carcinoma (SCC), the diagnosis of which can meet with many pitfalls: benign hyperplastic lesions and conventional SCC are the most important differential diagnoses. The microRNA miR-19a is overexpressed in many solid tumours and regulates the suppressor of cytokine signalling-1 (SOCS-1) expression. Aims: The main endpoints were to assess miR-19a and SOCS-1 expression in glottic VSCC, and the former's potential role in differentiating between glottic VSCC, conventional SCC and hyperplastic lesions. Methods: The expression of MiR-19a (by reverse transcription and quantitative real-time PCR) and SOCS-1 (by immunohistochemistry, rabbit polyclonal anti-SOCS-1 antibody) was assessed in 11 consecutive cases of glottic VSCC, 20 of papillary hyperplasia and 42 cases of conventional SCC. Results: Mean miR-19a expression was significantly higher (p = 0.000) in malignant glottic lesions (conventional SCC/VSCC) than in benign conditions. Significant differences in mean miR-19a expression also emerged between conventional SCC and papillary hyperplasia (p = 0.000), and between conventional SCC and VSCC (p = 0.03). miR-19a expression was not statistically associated with SOCS-1 immunoreactivity or immunostaining intensity in VSCC, conventional SCC or papillary hyperplasia. Conclusions: Our preliminary outcomes suggest the utility of miR-19a in the challenging differential diagnosis of laryngeal VSCC. Although miR-19a has been found to regulate SOCS-1 expression, this evidence was not confirmed by this investigation. (Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/)
Databáze:	MEDLINE
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