Investigation of the Viability, Adhesion, and Migration of Human Fibroblasts in a Hyaluronic Acid/Gelatin Microgel-Reinforced Composite Hydrogel for Vocal Fold Tissue Regeneration.

Autor: Heris HK; Biomechanics Laboratory, Department of Mechanical Engineering, Faculty of Engineering, McGill University, 817 Rue Sherbrooke Ouest, Montreal, QC, H3A 0C3, Canada., Daoud J; Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, QC, H3A 2B4, Canada., Sheibani S; Biological Threat Defence Section, Defence R&D Canada-Suffield, Station Main, Medicine Hat, AB, T1A 8K6, Canada.; Department of Anatomy and Cell Biology, Faculty of Medicine, McGill University, 3640 University Street, Montreal, QC, H3A 0C7, Canada., Vali H; Department of Anatomy and Cell Biology, Faculty of Medicine, McGill University, 3640 University Street, Montreal, QC, H3A 0C7, Canada., Tabrizian M; Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, QC, H3A 2B4, Canada.; Department of Anatomy and Cell Biology, Faculty of Medicine, McGill University, 3640 University Street, Montreal, QC, H3A 0C7, Canada., Mongeau L; Biomechanics Laboratory, Department of Mechanical Engineering, Faculty of Engineering, McGill University, 817 Rue Sherbrooke Ouest, Montreal, QC, H3A 0C3, Canada.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2016 Jan 21; Vol. 5 (2), pp. 255-65. Date of Electronic Publication: 2015 Oct 26.
DOI: 10.1002/adhm.201500370
Abstrakt: The potential use of a novel scaffold biomaterial consisting of cross-linked hyaluronic acid (HA)-gelatin (Ge) composite microgels is investigated for use in treating vocal fold injury and scarring. Cell adhesion integrins and kinematics of cell motion are investigated in 2D and 3D culture conditions, respectively. Human vocal fold fibroblast (hVFF) cells are seeded on HA-Ge microgels attached to a HA hydrogel thin film. The results show that hVFF cells establish effective adhesion to HA-Ge microgels through the ubiquitous expression of β1 integrin in the cell membrane. The microgels are then encapsulated in a 3D HA hydrogel for the study of cell migration. The cells within the HA-Ge microgel-reinforced composite hydrogel (MRCH) scaffold have an average motility speed of 0.24 ± 0.08 μm min(-1) . The recorded microscopic images reveal features that are presumably associated with lobopodial and lamellipodial cell migration modes within the MRCH scaffold. Average cell speed during lobopodial migration is greater than that during lamellipodial migration. The cells move faster in the MRCH than in the HA-Ge gel without microgels. These findings support the hypothesis that HA-Ge MRCH promotes cell adhesion and migration; thereby they constitute a promising biomaterial for vocal fold repair.
(© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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