An integrated linkage map reveals candidate genes underlying adaptive variation in Chinook salmon (Oncorhynchus tshawytscha).

Autor: McKinney GJ; School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA, 98195-5020, USA., Seeb LW; School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA, 98195-5020, USA., Larson WA; School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA, 98195-5020, USA., Gomez-Uchida D; School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA, 98195-5020, USA., Limborg MT; School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA, 98195-5020, USA., Brieuc MS; School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA, 98195-5020, USA., Everett MV; School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA, 98195-5020, USA., Naish KA; School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA, 98195-5020, USA., Waples RK; School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA, 98195-5020, USA., Seeb JE; School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA, 98195-5020, USA.
Jazyk: angličtina
Zdroj: Molecular ecology resources [Mol Ecol Resour] 2016 May; Vol. 16 (3), pp. 769-83. Date of Electronic Publication: 2015 Nov 25.
DOI: 10.1111/1755-0998.12479
Abstrakt: Salmonids are an important cultural and ecological resource exhibiting near worldwide distribution between their native and introduced range. Previous research has generated linkage maps and genomic resources for several species as well as genome assemblies for two species. We first leveraged improvements in mapping and genotyping methods to create a dense linkage map for Chinook salmon Oncorhynchus tshawytscha by assembling family data from different sources. We successfully mapped 14 620 SNP loci including 2336 paralogs in subtelomeric regions. This improved map was then used as a foundation to integrate genomic resources for gene annotation and population genomic analyses. We anchored a total of 286 scaffolds from the Atlantic salmon genome to the linkage map to provide a framework for the placement 11 728 Chinook salmon ESTs. Previously identified thermotolerance QTL were found to colocalize with several candidate genes including HSP70, a gene known to be involved in thermal response, as well as its inhibitor. Multiple regions of the genome with elevated divergence between populations were also identified, and annotation of ESTs in these regions identified candidate genes for fitness related traits such as stress response, growth and behaviour. Collectively, these results demonstrate the utility of combining genomic resources with linkage maps to enhance evolutionary inferences.
(© 2015 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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