The overexpression of an Amaranthus hypochondriacus NF-YC gene modifies growth and confers water deficit stress resistance in Arabidopsis.

Autor: Palmeros-Suárez PA; Centro de Investigación y de Estudios Avanzados del I. P. N. (Cinvestav), Unidad Irapuato, Km 9.6 del Libramiento Norte Carretera Irapuato-León, Apartado Postal 629, C.P. 36821 Irapuato, Guanajuato, Mexico., Massange-Sánchez JA; Centro de Investigación y de Estudios Avanzados del I. P. N. (Cinvestav), Unidad Irapuato, Km 9.6 del Libramiento Norte Carretera Irapuato-León, Apartado Postal 629, C.P. 36821 Irapuato, Guanajuato, Mexico., Martínez-Gallardo NA; Centro de Investigación y de Estudios Avanzados del I. P. N. (Cinvestav), Unidad Irapuato, Km 9.6 del Libramiento Norte Carretera Irapuato-León, Apartado Postal 629, C.P. 36821 Irapuato, Guanajuato, Mexico., Montero-Vargas JM; Centro de Investigación y de Estudios Avanzados del I. P. N. (Cinvestav), Unidad Irapuato, Km 9.6 del Libramiento Norte Carretera Irapuato-León, Apartado Postal 629, C.P. 36821 Irapuato, Guanajuato, Mexico., Gómez-Leyva JF; Laboratorio de Biología Molecular, Instituto Tecnológico de Tlajomulco, Jalisco (ITTJ), Km 10 Carretera a San Miguel Cuyutlán, C.P. 45640 Tlajomulco de Zúñiga, Jalisco, Mexico., Délano-Frier JP; Centro de Investigación y de Estudios Avanzados del I. P. N. (Cinvestav), Unidad Irapuato, Km 9.6 del Libramiento Norte Carretera Irapuato-León, Apartado Postal 629, C.P. 36821 Irapuato, Guanajuato, Mexico. Electronic address: jdelano@ira.cinvestav.mx.
Jazyk: angličtina
Zdroj: Plant science : an international journal of experimental plant biology [Plant Sci] 2015 Nov; Vol. 240, pp. 25-40. Date of Electronic Publication: 2015 Aug 20.
DOI: 10.1016/j.plantsci.2015.08.010
Abstrakt: Nuclear factor-Y (NF-Y), is a plant heterotrimeric transcription factor constituted by NF-YA, NF-YB and NF-YC subunits. The function of many NF-Y subunits, mostly of the A and B type, has been studied in plants, but knowledge regarding the C subunit remains fragmentary. Here, a water stress-induced NF-YC gene from Amaranthus hypochondriacus (AhNF-YC) was further characterized by its overexpression in transgenic Arabidospis thaliana plants. A role in development was inferred from modified growth rates in root, rosettes and inflorescences recorded in AhNF-YC overexpressing Arabidopsis plants, in addition to a delayed onset of flowering. Also, the overexpression of AhNF-YC caused increased seedling sensitivity to abscisic acid (ABA), and influenced the expression of several genes involved in secondary metabolism, development and ABA-related responses. An altered expression of the latter in water stressed and recovered transgenic plants, together with the observed increase in ABA sensitivity, suggested that their increased water stress resistance was partly ABA-dependent. An untargeted metabolomic analysis also revealed an altered metabolite pattern, both in normal and water stress/recovery conditions. These results suggest that AhNF-YC may play an important regulatory role in both development and stress, and represents a candidate gene for the engineering of abiotic stress resistance in commercial crops.
(Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.)
Databáze: MEDLINE
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