RNA Controls PolyQ Protein Phase Transitions.

Autor:	Zhang H; Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA; Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA., Elbaum-Garfinkle S; Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA., Langdon EM; Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA., Taylor N; Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA., Occhipinti P; Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA., Bridges AA; Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA., Brangwynne CP; Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA. Electronic address: cbrangwy@princeton.edu., Gladfelter AS; Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA. Electronic address: amy.gladfelter@dartmouth.edu.
Jazyk:	angličtina
Zdroj:	Molecular cell [Mol Cell] 2015 Oct 15; Vol. 60 (2), pp. 220-30.
DOI:	10.1016/j.molcel.2015.09.017
Abstrakt:	Compartmentalization in cells is central to the spatial and temporal control of biochemistry. In addition to membrane-bound organelles, membrane-less compartments form partitions in cells. Increasing evidence suggests that these compartments assemble through liquid-liquid phase separation. However, the spatiotemporal control of their assembly, and how they maintain distinct functional and physical identities, is poorly understood. We have previously shown an RNA-binding protein with a polyQ-expansion called Whi3 is essential for the spatial patterning of cyclin and formin transcripts in cytosol. Here, we show that specific mRNAs that are known physiological targets of Whi3 drive phase separation. mRNA can alter the viscosity of droplets, their propensity to fuse, and the exchange rates of components with bulk solution. Different mRNAs impart distinct biophysical properties of droplets, indicating mRNA can bring individuality to assemblies. Our findings suggest that mRNAs can encode not only genetic information but also the biophysical properties of phase-separated compartments. (Copyright © 2015 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu