| Autor: |
Ruseva B; Department of Physiology, Medical University, 1 Kliment Ohridski, 5800 Pleven, Bulgaria., Atanasova M; Department of Biology, Medical University, 1 Kliment Ohridski, 5800 Pleven, Bulgaria., Tsvetkova R; Department of Common and Clinical Pathology, Medical University, 1 Kliment Ohridski, 5800 Pleven, Bulgaria., Betova T; Department of Common and Clinical Pathology, Medical University, 1 Kliment Ohridski, 5800 Pleven, Bulgaria., Mollova M; Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, 73 Tsarigradsko Shose, 1113 Sofia, Bulgaria., Alexandrova M; Department of Biophysics, Medical University, 1 Kliment Ohridski, 5800 Pleven, Bulgaria., Laleva P; Central Clinical Laboratory of University Hospital, 8 Georgi Kochev, 5800 Pleven, Bulgaria., Dimitrova A; Department of Pathological Physiology, Medical University, 1 Kliment Ohridski, 5800 Pleven, Bulgaria. |
| Abstrakt: |
Selenium (Se) is an exogenous antioxidant that performs its function via the expression of selenoproteins. The aim of this study was to explore the effect of varying Se intake on the redox status of the aortic wall in young spontaneously hypertensive rats (SHR). Sixteen male Wistar Kyoto (WKY) rats and nineteen male SHR, 16-week-old, were tested after being given diets with different Se content for eight weeks. They were divided into 4 groups: control groups of WKY NSe and SHR NSe on an adequate Se diet and groups of WKY HSe and SHR HSe that received Se supplementation. The Se nutritional status was assessed by measuring whole blood glutathione peroxidase-1 (GPx-1) activity. Serum concentration of lipid hydroperoxides and serum level of antibodies against advanced glycation end products (anti-AGEs abs) were determined. Expression of GPx-1 and endothelial nitric oxide synthase (eNOS) were examined in aortic wall. Se supplementation significantly increased GPx-1 activity of whole blood and in the aortas of WKY and SHR. Decreased lipid peroxidation level, eNOS-3 expression in the aortic wall, and serum level of anti-AGEs abs were found in SHR HSe compared with SHR NSe. In conclusion, Se supplementation improved the redox status of the aortic wall in young SHR. |
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