[INTRA-ERYTHROCYTE INVASION OF ESCHERICHIA COLI STRAINS WITH VARIOUS LEVELS OF ANTI-HEMOGLOBIN ACTIVITY IN EXPERIMENT].

Autor:	Schuplova EA, Fadeev SB, Bukharin OV
Jazyk:	ruština
Zdroj:	Zhurnal mikrobiologii, epidemiologii i immunobiologii [Zh Mikrobiol Epidemiol Immunobiol] 2015 Jul-Aug (4), pp. 40-4.
Abstrakt:	Aim: Study of intra-erythrocyte invasion of Escherichia coli strains with various levels of anti-hemoglobin activity on a model of generalized infection. Materials and Methods: Experiments were carried out on 72 male mice. Generalized infection was induced by intravenous administration of E. coli strain day culture suspension with various values of hemolytic and anti-hemoglobin activity. The levels of hemoglobin in animal blood was determined by hemoglobin-cyanide method. Intra-erythrocyte invasion of E.coli was studied by using laser scanning confocal microscopy (LSCM). Results: The largest decline (p < 0.05) of hemoglobin levels was detected in animals, infected with E. coli strains with a high levels of antiHbA and HA (58.3 ± 0.6 g/l and 69.7 ± 1.1 g/l). Using LSCM, microogranisms with a high levels of antiHbA were established to penetrate erythrocytes more frequently (70 ± 3.4%), than with a low (5.5 ± 1.7%). Conclusion: The largest frequency of bacteria detection in erythrocytes and the highest decline of blood hemoglobin levels were noted during infection of animals with E. coli strains with high anti-hemoglobin activity.
Databáze:	MEDLINE
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