Enhancing the Quality of Antibodies to HIV-1 Envelope by GagPol-Specific Th Cells.

Autor:	Storcksdieck genannt Bonsmann M; Department of Molecular and Medical Virology, Ruhr-University Bochum, Bochum 44801, Germany;, Niezold T; Department of Molecular and Medical Virology, Ruhr-University Bochum, Bochum 44801, Germany;, Temchura V; Department of Molecular and Medical Virology, Ruhr-University Bochum, Bochum 44801, Germany; Universitätsklinikum Erlangen, Institut für Klinische und Molekulare Virologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen 91054, Germany;, Pissani F; U.S. Military HIV Research Program, Silver Spring, MD 20910;, Ehrhardt K; Department of Medical Microbiology and Hygiene, Institute of Virology, University Medical Center Freiburg, Freiburg 79106, Germany;, Brown EP; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755; and., Osei-Owusu NY; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755; and., Hannaman D; Ichor Medical Systems, Inc., San Diego, CA 92121., Hengel H; Department of Medical Microbiology and Hygiene, Institute of Virology, University Medical Center Freiburg, Freiburg 79106, Germany;, Ackerman ME; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755; and., Streeck H; U.S. Military HIV Research Program, Silver Spring, MD 20910;, Nabi G; Department of Molecular and Medical Virology, Ruhr-University Bochum, Bochum 44801, Germany;, Tenbusch M; Department of Molecular and Medical Virology, Ruhr-University Bochum, Bochum 44801, Germany;, Überla K; Department of Molecular and Medical Virology, Ruhr-University Bochum, Bochum 44801, Germany; Universitätsklinikum Erlangen, Institut für Klinische und Molekulare Virologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen 91054, Germany; klaus.ueberla@fau.de.
Jazyk:	angličtina
Zdroj:	Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2015 Nov 15; Vol. 195 (10), pp. 4861-72. Date of Electronic Publication: 2015 Oct 14.
DOI:	10.4049/jimmunol.1501377
Abstrakt:	The importance of Fc-dependent effector functions of Abs induced by vaccination is increasingly recognized. However, vaccination of mice against HIV envelope (Env) induced a skewed Th cell response leading to Env-specific Abs with reduced effector function. To overcome this bias, GagPol-specific Th cells were harnessed to provide intrastructural help for Env-specific B cells after immunization with virus-like particles containing GagPol and Env. This led to a balanced Env-specific humoral immune response with a more inflammatory Fc glycan profile. The increased quality in the Ab response against Env was confirmed by FcγR activation assays. Because the Env-specific Th cell response was also biased in human vaccinees, intrastructural help is an attractive novel approach to increase the efficacy of prophylactic HIV Env-based vaccines and may also be applicable to other particulate vaccines. (Copyright © 2015 by The American Association of Immunologists, Inc.)
Databáze:	MEDLINE
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