| Autor: |
Mellbye FB; Department of Endocrinology and Internal Medicine, Aarhus University Hospital , Tage-Hansens Gade 2, 8000 Aarhus C Denmark., Jeppesen PB; Department of Endocrinology and Internal Medicine, Aarhus University Hospital , Tage-Hansens Gade 2, 8000 Aarhus C Denmark., Hermansen K; Department of Endocrinology and Internal Medicine, Aarhus University Hospital , Tage-Hansens Gade 2, 8000 Aarhus C Denmark., Gregersen S; Department of Endocrinology and Internal Medicine, Aarhus University Hospital , Tage-Hansens Gade 2, 8000 Aarhus C Denmark. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of natural products [J Nat Prod] 2015 Oct 23; Vol. 78 (10), pp. 2447-51. Date of Electronic Publication: 2015 Oct 14. |
| DOI: |
10.1021/acs.jnatprod.5b00481 |
| Abstrakt: |
Diet and exercise intervention can delay or prevent development of type-2-diabetes (T2D), and high habitual coffee consumption is associated with reduced risk of developing T2D. This study aimed to test whether selected bioactive substances in coffee acutely and/or chronically increase insulin secretion from β-cells and improve insulin sensitivity in skeletal muscle cells. Insulin secretion from INS-1E rat insulinoma cells was measured after acute (1-h) and long-term (72-h) incubation with bioactive substances from coffee. Additionally, we measured uptake of radioactive glucose in human skeletal muscle cells (SkMC) after incubation with cafestol. Cafestol at 10(-8) and 10(-6) M acutely increased insulin secretion by 12% (p < 0.05) and 16% (p < 0.001), respectively. Long-term exposure to 10(-10) and 10(-8) M cafestol increased insulin secretion by 34% (p < 0.001) and 68% (p < 0.001), respectively. Caffeic acid also increased insulin secretion acutely and chronically. Chlorogenic acid, trigonelline, oxokahweol, and secoisolariciresinol did not significantly alter insulin secretion acutely. Glucose uptake in SkMC was significantly enhanced by 8% (p < 0.001) in the presence of 10(-8) M cafestol. This newly demonstrated dual action of cafestol suggests that cafestol may contribute to the preventive effects on T2D in coffee drinkers and be of therapeutic interest. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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