Exploration of intrinsic and extrinsic apoptotic pathways in zearalenone-treated rat sertoli cells.

Autor: Xu ML; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China., Hu J; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China., Guo BP; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China., Niu YR; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China., Xiao C; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China., Xu YX; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Jazyk: angličtina
Zdroj: Environmental toxicology [Environ Toxicol] 2016 Dec; Vol. 31 (12), pp. 1731-1739. Date of Electronic Publication: 2015 Oct 13.
DOI: 10.1002/tox.22175
Abstrakt: Zearalenone (ZEA) is a nonsteroidal estrogenic mycotoxin produced mainly by Fusarium. ZEA causes reproductive disorders and is both cytotoxic and genotoxic in animals; however, little is known regarding the molecular mechanism(s) leading to ZEA toxicity. Sertoli cells are somatic cells that support the development of spermatogenic cells. The objective of this study was to explore the effects of ZEA on the proliferation, apoptosis, and necrosis of rat Sertoli cells to uncover signaling pathways underlying ZEA cytotoxicity. ZEA reduced the proliferation of rat Sertoli cells in a dose-dependent manner, as indicated by a CCK8 assay, while flow cytometry revealed that ZEA caused both apoptosis and necrosis. Immunoblotting revealed that ZEA treatment increased the ratio of Bax/Bcl-2, as well as the expression of FasL and caspases-3, -8, and -9, in a dose-dependent manner. Collectively, these data suggest that ZEA induced apoptosis and necrosis in rat Sertoli cells via extrinsic and intrinsic apoptotic pathways. This study provides new insights into the molecular mechanisms by which ZEA exhibits cytotoxicity. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1731-1739, 2016.
(© 2015 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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