Synthesis, Characterization, and Biological Activities of Pendant Arm-Pyridyltetrazole Copper(II) Complexes: DNA Binding/Cleavage Activity and Cytotoxic Studies.

Autor: Mustafa S; Department of Chemistry, GITAM University Hyderabad campus, Hyderabad 502329, India., Rao BU; Department of Inorganic and Analytical Chemistry, Andhra University, Visakhapatnam, India., Surendrababu MS; Department of Chemistry, GITAM University Hyderabad campus, Hyderabad 502329, India. manabolu@gmail.com., Raju KK; Department of Biotechnology, Gokaraju Rangaraju Institute of Technology, Hyderabad, India., Rao GN; Department of Inorganic and Analytical Chemistry, Andhra University, Visakhapatnam, India.
Jazyk: angličtina
Zdroj: Chemistry & biodiversity [Chem Biodivers] 2015 Oct; Vol. 12 (10), pp. 1516-34.
DOI: 10.1002/cbdv.201400369
Abstrakt: 2-(1H-Tetrazol-5-yl)pyridine (L) has been reacted separately with Me2NCH2CH2Cl⋅HCl and ClCH2CH2OH to yield two regioisomers in each case, N,N-dimethyl-2-[5-(pyridin-2-yl)-1H-tetrazol-1-yl]ethanamine (L1)/N,N-dimethyl-2-[5-(pyridin-2-yl)-2H-tetrazol-2-yl]ethanamine (L2) and 2-[5-(pyridin-2-yl)-1H-tetrazol-1-yl]ethanol (L3)/2-[5-(pyridin-2-yl)-2H-tetrazol-2-yl]ethanol (L4), respectively. These ligands, L1-L4, have been coordinated with CuCl2 ⋅H2O in 1 : 1 composition to furnish the corresponding complexes 1-4. EPR Spectra of Cu complexes 1 and 3 were characteristic of square planar geometry, with nuclear hyperfine spin 3/2. Single X-ray crystallographic studies of 3 revealed that the Cu center has a square planar structure. DNA binding studies were carried out by UV/VIS absorption; viscosity and thermal denaturation studies revealed that each of these complexes are avid binders of calf thymus DNA. Investigation of nucleolytic cleavage activities of the complexes was carried out on double-stranded pBR322 circular plasmid DNA by using a gel electrophoresis experiment under various conditions, where cleavage of DNA takes place by oxidative free-radical mechanism (OH(⋅)). In vitro anticancer activities of the complexes against MCF-7 (human breast adenocarcinoma) cells revealed that the complexes inhibit the growth of cancer cells. The IC50 values of the complexes showed that Cu complexes exhibit comparable cytotoxic activities compared to the standard drug cisplatin.
(Copyright © 2015 Verlag Helvetica Chimica Acta AG, Zürich.)
Databáze: MEDLINE
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