Immobilization of pectin depolymerising polygalacturonase using different polymers.

Autor: Ur Rehman H; Department of Chemistry, University of Turbat, Kech, Balochistan, Pakistan. Electronic address: rehman.haneef8@gmail.com., Aman A; The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan., Nawaz MA; Department of Biotechnology, Shaheed Benazir Bhutto University, Sheringal, Dir (Upper), KPK, Pakistan., Karim A; The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan., Ghani M; The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan., Baloch AH; Faculty of Agriculture, Lasbela University of Agriculture, Water and Marine Sciences (LUAWMS), Uthal 90150, Balochistan, Pakistan., Ul Qader SA; The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2016 Jan; Vol. 82, pp. 127-33. Date of Electronic Publication: 2015 Oct 25.
DOI: 10.1016/j.ijbiomac.2015.10.012
Abstrakt: Polygalacturonase catalyses the hydrolysis of pectin substances and widely has been used in food and textile industries. In current study, different polymers such as calcium alginate beads, polyacrylamide gel and agar-agar matrix were screened for the immobilization of polygalacturonase through entrapment technique. Polyacrylamide gel was found to be most promising one and gave maximum (89%) immobilization yield as compared to agar-agar (80%) and calcium alginate beads (46%). The polymers increased the reaction time of polygalacturonase and polymers entrapped polygalacturonases showed maximum pectinolytic activity after 10 min of reaction as compared to free polygalacturonase which performed maximum activity after 5.0 min of reaction time. The temperature of polygalacturonase for maximum enzymatic activity was increased from 45°C to 50°C and 55°C when it was immobilized within agar-agar and calcium alginate beads, respectively. The optimum pH (pH 10) of polygalacturonase was remained same when it was immobilized within polyacrylamide gel and calcium alginate beads, but changed from pH 10 to pH 9.0 after entrapment within agar-agar. Thermal stability of polygalacturonase was improved after immobilization and immobilized polygalacturonases showed higher tolerance against different temperatures as compared to free enzyme. Polymers entrapped polygalacturonases showed good reusability and retained more than 80% of their initial activity during 2nd cycles.
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Databáze: MEDLINE