Isolation and characterization of major histocompatibility complex class II B genes in cranes.

Autor: Kohyama TI; Department of Natural History Sciences, Faculty of Science, Hokkaido University, N10W8, Kita-ku, Sapporo, Hokkaido, 060-0810, Japan., Akiyama T; Department of Natural History Sciences, Faculty of Science, Hokkaido University, N10W8, Kita-ku, Sapporo, Hokkaido, 060-0810, Japan., Nishida C; Department of Natural History Sciences, Faculty of Science, Hokkaido University, N10W8, Kita-ku, Sapporo, Hokkaido, 060-0810, Japan., Takami K; Osaka Municipal Tennoji Zoological Gardens, 1-108, Chausuyama-cho, Tennoji-ku, Osaka, Osaka, 543-0063, Japan., Onuma M; Ecological Genetics Analysis Section, Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, 16-2, Onogawa, Tsukuba, Ibaraki, 305-8506, Japan., Momose K; Red-crowned Crane Conservancy, Wakatake-cho, Kushiro, Hokkaido, 085-0036, Japan., Masuda R; Department of Natural History Sciences, Faculty of Science, Hokkaido University, N10W8, Kita-ku, Sapporo, Hokkaido, 060-0810, Japan. masudary@mail.sci.hokudai.ac.jp.
Jazyk: angličtina
Zdroj: Immunogenetics [Immunogenetics] 2015 Nov; Vol. 67 (11-12), pp. 705-10. Date of Electronic Publication: 2015 Oct 09.
DOI: 10.1007/s00251-015-0874-x
Abstrakt: In this study, we isolated and characterized the major histocompatibility complex (MHC) class II B genes in cranes. Genomic sequences spanning exons 1 to 4 were amplified and determined in 13 crane species and three other species closely related to cranes. In all, 55 unique sequences were identified, and at least two polymorphic MHC class II B loci were found in most species. An analysis of sequence polymorphisms showed the signature of positive selection and recombination. A phylogenetic reconstruction based on exon 2 sequences indicated that trans-species polymorphism has persisted for at least 10 million years, whereas phylogenetic analyses of the sequences flanking exon 2 revealed a pattern of concerted evolution. These results suggest that both balancing selection and recombination play important roles in the crane MHC evolution.
Databáze: MEDLINE
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