| Autor: |
Hayashi G; a Research Reactor Institute; Kyoto University ; Osaka , Japan.; b Institute of Development; Aging and Cancer; Tohoku University ; Sendai , Japan., Moro CF; c Department of Anatomy ; School of Medicine; Showa University ; Tokyo , Japan., Rohila JS; d Department of Biology and Microbiology ; South Dakota State University ; SD USA., Shibato J; c Department of Anatomy ; School of Medicine; Showa University ; Tokyo , Japan.; e Global Research Center for Innovative Life Science; School of Pharmacy and Pharmaceutical Sciences; Hoshi University ; Tokyo , Japan., Kubo A; f Environmental Stress Mechanisms Section; Center for Environmental Biology and Ecosystem Studies; National Institute for Environmental Studies ; Tsukuba, Ibaraki , Japan., Imanaka T; a Research Reactor Institute; Kyoto University ; Osaka , Japan., Kimura S; g Laboratory of International Epidemiology; Center for International Cooperation; Dokkyo Medical University ; Tochigi , Japan., Ozawa S; h 1372-7 Kusabana ; Tokyo , Japan., Fukutani S; a Research Reactor Institute; Kyoto University ; Osaka , Japan., Endo S; i Department of Quantam Energy Applications ; Graduate School of Engineering; Hiroshima University ; Higashi-Hiroshima , Japan., Ichikawa K; j Office Brain ; Tokyo , Japan., Agrawal GK; k Research Laboratory for Biotechnology and Biochemistry (RLABB) ; Kathmandu , Nepal.; l GRADE (Global Research Arch for Developing Education) Academy Pvt. Ltd ; Birgunj , Nepal., Shioda S; c Department of Anatomy ; School of Medicine; Showa University ; Tokyo , Japan.; e Global Research Center for Innovative Life Science; School of Pharmacy and Pharmaceutical Sciences; Hoshi University ; Tokyo , Japan., Hori M; m School of Pharmacy; Showa University ; Tokyo , Japan., Fukumoto M; b Institute of Development; Aging and Cancer; Tohoku University ; Sendai , Japan., Rakwal R; c Department of Anatomy ; School of Medicine; Showa University ; Tokyo , Japan.; e Global Research Center for Innovative Life Science; School of Pharmacy and Pharmaceutical Sciences; Hoshi University ; Tokyo , Japan.; n Faculty of Health and Sport Sciences & Tsukuba International Academy for Sport Studies (TIAS); University of Tsukuba ; Tsukuba , Ibaraki , Japan. |
| Abstrakt: |
The present study continues our previous research on investigating the biological effects of low-level gamma radiation in rice at the heavily contaminated Iitate village in Fukushima, by extending the experiments to unraveling the leaf proteome. 14-days-old plants of Japonica rice (Oryza sativa L. cv. Nipponbare) were subjected to gamma radiation level of upto 4 µSv/h, for 72 h. Following exposure, leaf samples were taken from the around 190 µSv/3 d exposed seedling and total proteins were extracted. The gamma irradiated leaf and control leaf (harvested at the start of the experiment) protein lysates were used in a 2-D differential gel electrophoresis (2D-DIGE) experiment using CyDye labeling in order to asses which spots were differentially represented, a novelty of the study. 2D-DIGE analysis revealed 91 spots with significantly different expression between samples (60 positive, 31 negative). MALDI-TOF and TOF/TOF mass spectrometry analyses revealed those as comprising of 59 different proteins (50 up-accumulated, 9 down-accumulated). The identified proteins were subdivided into 10 categories, according to their biological function, which indicated that the majority of the differentially expressed proteins consisted of the general (non-energy) metabolism and stress response categories. Proteome-wide data point to some effects of low-level gamma radiation exposure on the metabolism of rice leaves. |
