Assessment of Parasite Liver-Stage Burden in Human-Liver Chimeric Mice.

Autor:	Foquet L; Center for Vaccinology, Ghent University and University Hospital, De Pintelaan 185, Ghent, 9000, Belgium., Meuleman P; Center for Vaccinology, Ghent University and University Hospital, De Pintelaan 185, Ghent, 9000, Belgium., Hermsen CC; Medical Centre, Radboud University Nijmegen, Geert Grooteplein 28, GA 6525, Nijmegen, The Netherlands., Sauerwein R; Medical Centre, Radboud University Nijmegen, Geert Grooteplein 28, GA 6525, Nijmegen, The Netherlands., Leroux-Roels G; Center for Vaccinology, Ghent University and University Hospital, De Pintelaan 185, Ghent, 9000, Belgium. geert.lerouxroels@ugent.be.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2015; Vol. 1325, pp. 59-68.
DOI:	10.1007/978-1-4939-2815-6_5
Abstrakt:	Humanized mice with a chimeric liver are a promising tool to evaluate the "in vivo" efficacy of novel compounds or vaccine-induced antibodies directed against the pre-erythrocytic stages of Plasmodium falciparum. The absence of human red blood cells in these humanized mice precludes the transition from liver to blood stage. The qPCR-based method described below allows for a sensitive and reliable quantification of parasite DNA in the chimeric liver following a challenge via infected mosquito bite or intravenous injection of sporozoites. With this method approximately 25 % of the total chimeric liver is examined and a single infected hepatocyte can be detected in the analyzed tissue. The use of appropriate species-specific probes can also allow for the detection of other Plasmodium species in vivo.
Databáze:	MEDLINE
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