Ploidy and domestication are associated with genome size variation in Palms.

Autor: Gunn BF; Research School of Biology, Division of Evolution, Ecology and Genetics, The Australian National University, Acton, ACT 2601, Australia bee.gunn@anu.edu.au., Baudouin L; CIRAD, UMR AGAP, F-34398 Montpellier, France., Beulé T; CIRAD, UMR DIADE, F-34398 Montpellier, France., Ilbert P; CIRAD, UMR DIADE, F-34398 Montpellier, France., Duperray C; Montpellier Rio Imaging, IRB, INSERM U1040, CHU de Montpellier 34295 Montpellier, France., Crisp M; Research School of Biology, Division of Evolution, Ecology and Genetics, The Australian National University, Acton, ACT 2601, Australia., Issali A; Station de Recherche Marc Delorme, Port Bouët. 07 BP 13 Abidjan, Côte d'Ivoire., Konan JL; Station de Recherche Marc Delorme, Port Bouët. 07 BP 13 Abidjan, Côte d'Ivoire., Rival A; CIRAD, UMR DIADE, F-34398 Montpellier, France.
Jazyk: angličtina
Zdroj: American journal of botany [Am J Bot] 2015 Oct; Vol. 102 (10), pp. 1625-33. Date of Electronic Publication: 2015 Oct 05.
DOI: 10.3732/ajb.1500164
Abstrakt: Premise of the Study: The genome size of a species (C-value) is associated with growth, development and adaptation to environmental changes. Angiosperm C-values range 1200-fold and frequently vary within species, although little is known about the impacts of domestication on genome size. Genome size variation among related species of palms is of evolutionary significance because changes characterize clades and may be associated with polyploidy, transposon amplifications, deletions, or rearrangements. Further knowledge of genome size will provide crucial information needed for planning of whole genome sequencing and accurate annotations. We studied the genome size of Cocos nucifera and its variation among cultivars, and compared it to values for related palms from the Attaleinae subtribe.
Methods: Flow cytometric analysis of isolated nuclei from young palm leaves was used to estimate genome sizes of 23 coconut cultivars (Talls, Dwarfs, and hybrids) worldwide and 17 Cocoseae species. Ancestral genome size was reconstructed on a maximum likelihood phylogeny of Attaleinae from seven WRKY loci.
Key Results: The coconut genome is large-averaging 5.966 pg-and shows intraspecific variation associated with domestication. Variation among Tall coconuts was significantly greater than among Dwarfs. Attaleinae genomes showed moderate size variation across genera, except polyploids Jubaeopsis caffra, Voanioala gerardii, Beccariophoenix alfredii, and Allagoptera caudescens, which had larger genomes.
Conclusions: Our results contribute to the understanding of the relationship between domestication and genome size in long-lived tree crops and provide a basis for whole-genome sequencing of the coconut and other domesticated plants. Polyploidy evolved independently in two clades within Attaleinae.
(© 2015 Botanical Society of America.)
Databáze: MEDLINE
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