Induction of Liver Tumors in Mice with N-Ethyl-N-Nitrosourea or N-Nitrosodiethylamine.
| Autor: | Gurley KE; Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109., Moser RD; Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109., Kemp CJ; Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109. |
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| Jazyk: | angličtina |
| Zdroj: | Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2015 Oct 01; Vol. 2015 (10), pp. 941-2. Date of Electronic Publication: 2015 Oct 01. |
| DOI: | 10.1101/pdb.prot077438 |
| Abstrakt: | Since the groundbreaking studies in the middle part of the last century showing liver cancer in rodents exposed to aromatic amines, the liver has been widely used as a model target organ of chemical carcinogenesis. This protocol describes a method for inducing liver tumors by injecting mice with the widely used alkylating agents N-ethyl-N-nitrosourea (ENU) and N-nitrosodiethylamine (DEN). ENU does not require metabolic activation and readily induces tumors in a number of tissues, including the lungs, stomach, and ovaries, as well as inducing lymphomas. Mice injected with DEN can also develop other tumors, including those of the gastrointestinal tract, skin, lungs, and lymphocytes, but because DEN is metabolized in the liver, it is most effective at inducing liver tumors. (© 2015 Cold Spring Harbor Laboratory Press.) |
| Databáze: | MEDLINE |
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