Phylogenetic analysis of Indian rabies virus isolates targeting the complete glycoprotein gene.

Autor: Cherian S; Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P. 243 122, India. Electronic address: cpasusan18@gmail.com., Singh R; Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P. 243 122, India. Electronic address: rajendra_singh5747@rediffmail.com., Singh KP; CARDRAD, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P. 243 122, India., Manjunatha Reddy GB; NIVEDI, Bengaluru, Karnataka 560 064, India., Anjaneya; Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P. 243 122, India., Ravi Kumar GVPPS; Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P. 243 122, India., Sumithra TG; Department of Veterinary Microbiology, College of Veterinary And Animal Sciences, Pookot, Wayanad, Kerala 673 576, India., Singh RP; Division of Biological Products, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P. 243 122, India.
Jazyk: angličtina
Zdroj: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2015 Dec; Vol. 36, pp. 333-338. Date of Electronic Publication: 2015 Sep 30.
DOI: 10.1016/j.meegid.2015.09.024
Abstrakt: Rabies a fatal viral zoonosis is endemic in India. There is no report on phylogenetic study of Indian rabies virus isolates based on the complete G gene. In the present study, a total of 25 rabies positive brain samples collected during 2001-2014 from North India (UP, MP, Delhi, Rajasthan), South India (Kerala and Karnataka) and Gujarat states belonging to six different host species were subjected to G gene amplification by RT-PCR as three overlapping fragments of 881 bp, 991 bp and 618 bp. Phylogenetic analysis revealed that all Indian rabies virus isolates are genetically closely related with Arctic-like 1a lineage viruses. However, two distinct clusters were identified namely, India South and India North. All the Indian rabies isolates had 95.5-100% homology related to geography, but not to host species. Deduced amino acids on comparison revealed two amino acid changes, aa 356 in ECTO; N→K and aa 458; M→I, which were found to distinguish between the India South and India North isolates.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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