MC3T3-E1 Cells Behavior on Surfaces Bombarded by Argon Ions in Planar Cathode Discharge.

Autor: Moura CE; Department of Animal Science, Federal University of Semiarid Region, Mossoró, RN, Brazil., Silva NB; Department of Animal Science, Federal University of Semiarid Region, Mossoró, RN, Brazil., Sa JC; Department of Mechanical Engineering, Federal University of Rio Grande do Norte, Natal, RN, Brazil., Cavalcanti GB Junior; Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte, Natal, RN, Brazil., de Medeiros SR; Department of Cellular Biology and Genetics, Federal University of Rio Grande do Norte, Natal, RN, Brazil., Rocha HA; Department of Biochemistry, Federal University of Rio Grande do Norte, Natal, RN., Papa PC; Department of Surgery, University of São Paulo, São Paulo, Brazil., Alves C Jr; Department of Mechanical Engineering, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
Jazyk: angličtina
Zdroj: Artificial organs [Artif Organs] 2016 May; Vol. 40 (5), pp. 497-504. Date of Electronic Publication: 2015 Sep 29.
DOI: 10.1111/aor.12597
Abstrakt: To evaluate the effect of topography in nanoscale, titanium surfaces were bombarded by argon ions (a chemically inert gas), in an atmosphere of plasma. The effects of surface parameters on morphology, adhesion, proliferation, and MC3T3-E1 preosteoblasts differentiation were analyzed. Nontreated (smooth) surfaces were used as a control. The levels of average roughness (Ra) observed in bombarded and smooth titanium surfaces were of 95 and 14 nm, respectively. The wettability increased on treated surfaces. The number of attached cells (30 and 60 min) was significantly higher on the bombarded surface. The cell proliferation after 3 and 7 days was also significantly higher on the ion-bombarded surface. In addition, the ALP activity and expression of osteocalcin were higher in cells grown on the treated surface. The results showed that bombardment with argon ions increased the roughness and the wettability of the Ti surface, promoting a significant increase in the adhesion, proliferation, and differentiation of preosteoblasts.
(Copyright © 2015 International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.)
Databáze: MEDLINE
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