| Autor: |
Souza TS; Universidade Federal do Recôncavo da Bahia, Cruz das Almas, BA, Brazil ., Pinheiro RR; Empresa Brasileira de Pesquisa Agropecuária, Embrapa Caprinos e Ovinos, Sobral, CE, Brazil ., Costa JN; Universidade Federal do Recôncavo da Bahia, Cruz das Almas, BA, Brazil ., Lima CC; Universidade Federal da Bahia, Salvador, BA, Brazil ., Andrioli A; Empresa Brasileira de Pesquisa Agropecuária, Embrapa Caprinos e Ovinos, Sobral, CE, Brazil ., Azevedo DA; Universidade Estadual Vale do Acaraú, Sobral, CE, Brazil ., Santos VW; Universidade Estadual Vale do Acaraú, Sobral, CE, Brazil ., Araújo JF; Universidade Estadual Vale do Acaraú, Sobral, CE, Brazil ., Sousa AL; Universidade Estadual Vale do Acaraú, Sobral, CE, Brazil ., Pinheiro DN; Universidade Federal do Recôncavo da Bahia, Cruz das Almas, BA, Brazil ., Fernandes FM; Universidade Federal da Bahia, Salvador, BA, Brazil ., Costa Neto AO; Universidade Estadual de Feira de Santana, Feira de Santana, BA, Brazil . |
| Abstrakt: |
This study was conducted in order to evaluate the transmission of caprine lentivirus to sheep using different experimental groups. The first one (colostrum group) was formed by nine lambs receiving colostrum from goats positive for small ruminant lentiviruses (SRLV). The second group (milk group) was established by nine lambs that received milk of these goats. Third was a control group, consisting of lambs that suckled colostrum and milk of negative mothers. Another experimental group (contact group) was formed by eight adult sheep, confined with two naturally infected goats. The groups were monitored by immunoblotting (IB), enzyme-linked immunosorbent assay (ELISA), agar gel immunodiffusion (AGID) and nested polymerase chain reaction (nPCR). All lambs that suckled colostrum and milk of infected goats and six sheep of the contact group had positive results in the nPCR, although seroconversion was detected only in three of the exposed animals, with no clinical lentiviruses manifestation, in 720 days of observation. There was a close relationship between viral sequences obtained from infected animals and the prototype CAEV-Cork. Thus, it was concluded that SRLV can be transmitted from goats to sheep, however, the degree of adaptation of the virus strain to the host species probably interferes with the infection persistence and seroconversion rate. |
