Long-term evaluation of oral gavage with periodontopathogens or ligature induction of experimental periodontal disease in mice.

Autor: de Molon RS; Division of Diagnostic and Surgical Sciences, UCLA School of Dentistry, 10833 Le Conte Ave, Los Angeles, CA, 90095, USA. molon.foar@yahoo.com.br.; Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Sao Paulo State University, Rua Humaita, 1680, Centro, 14801-903, Araraquara, SP, Brazil. molon.foar@yahoo.com.br., Mascarenhas VI; Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Sao Paulo State University, Rua Humaita, 1680, Centro, 14801-903, Araraquara, SP, Brazil., de Avila ED; Department of Dental Materials and Prosthodontics, School of Dentistry at Araraquara, Sao Paulo State University, Rua Humaita, 1680, Centro, 14801-903, Araraquara, SP, Brazil., Finoti LS; Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Sao Paulo State University, Rua Humaita, 1680, Centro, 14801-903, Araraquara, SP, Brazil., Toffoli GB; Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Sao Paulo State University, Rua Humaita, 1680, Centro, 14801-903, Araraquara, SP, Brazil., Spolidorio DM; Department of Physiology and Pathology, School of Dentistry at Araraquara, Sao Paulo State University, Rua Humaita, 1680, Centro, 14801-903, Araraquara, SP, Brazil., Scarel-Caminaga RM; Department of Morphology, School of Dentistry at Araraquara, Sao Paulo State University, Rua Humaita, 1680, Centro, 14801-903, Araraquara, SP, Brazil., Tetradis S; Division of Diagnostic and Surgical Sciences, UCLA School of Dentistry, 10833 Le Conte Ave, Los Angeles, CA, 90095, USA.; Molecular Biology Institute, UCLA, Los Angeles, CA, 90095, USA., Cirelli JA; Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Sao Paulo State University, Rua Humaita, 1680, Centro, 14801-903, Araraquara, SP, Brazil. cirelli@foar.unesp.br.; School of Dentistry at Araraquara, Universidade Estadual Paulista (UNESP), Rua Humaitá, 1680, Araraquara, SP, 14801-903, Brazil. cirelli@foar.unesp.br.
Jazyk: angličtina
Zdroj: Clinical oral investigations [Clin Oral Investig] 2016 Jul; Vol. 20 (6), pp. 1203-16. Date of Electronic Publication: 2015 Sep 28.
DOI: 10.1007/s00784-015-1607-0
Abstrakt: Objective: To evaluate in long-term periods the destruction of periodontal tissues and bacterial colonization induced by oral gavage with periodontopathogens or ligature experimental periodontal disease models.
Material and Methods: Forty-eight C57BL/6 J mice were divided into four groups: group C: negative control; group L: ligature; group G-Pg: oral gavage with Porphyromonas gingivalis; and group G-PgFn: oral gavage with Porphyromonas gingivalis associated with Fusobacterium nucleatum. Mice were infected by oral gavage five times in 2-day intervals. After 45 and 60 days, animals were sacrificed and the immune-inflammatory response in the periodontal tissue was assessed by stereometric analysis. The alveolar bone loss was evaluated by live microcomputed tomography and histometric analysis. qPCR was used to confirm the bacterial colonization in all the groups. Data were analyzed using the Kruskal-Wallis, Wilcoxon, and ANOVA tests, at 5 % of significance level.
Results: Ligature model induced inflammation and bone resorption characterized by increased number of inflammatory cells and decreased number of fibroblasts, followed by advanced alveolar bone loss at 45 and 60 days (p < 0.05). Bacterial colonization in groups G-Pg and G-PgFn was confirmed by qPCR but inflammation and bone resorption were not observed (p < 0.05).
Conclusions: The ligature model but not the oral gavage models were effective to induce inflammation and bone loss in long-term periods. Pg colonization was observed in all models of experimental periodontal disease induction, independent of tissue alterations. These mice models of periodontitis validates, compliments, and enhances published PD models that utilize ligature or oral gavage and supports the importance of a successful colonization of a susceptible host, a bacterial invasion into vulnerable tissue, and host-bacterial interactions that lead to tissue destruction.
Clinical Relevance: The ligature model was an effective approach to induce inflammation and bone loss similar to human periodontitis, but the oral gavage models were not efficient in inducing periodontal inflammation and tissue destruction in the conditions studied. Ligature models can provide a basis for future interventional studies that contribute to the understanding of the disease pathogenesis and the complex host response to microbial challenge.
Databáze: MEDLINE
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