How many DNA markers are needed to reveal cryptic fungal species?

Autor: Balasundaram SV; Section for Genetics and Evolutionary Biology (EVOGENE), Department of Biosciences, University of Oslo, P.O. Box 1066 Blindern, 0316 Oslo, Norway. Electronic address: sudhagar@ibv.uio.no., Engh IB; Section for Genetics and Evolutionary Biology (EVOGENE), Department of Biosciences, University of Oslo, P.O. Box 1066 Blindern, 0316 Oslo, Norway. Electronic address: Ingeborg.Bjorvand.Engh@mycoteam.no., Skrede I; Section for Genetics and Evolutionary Biology (EVOGENE), Department of Biosciences, University of Oslo, P.O. Box 1066 Blindern, 0316 Oslo, Norway. Electronic address: inger.skrede@ibv.uio.no., Kauserud H; Section for Genetics and Evolutionary Biology (EVOGENE), Department of Biosciences, University of Oslo, P.O. Box 1066 Blindern, 0316 Oslo, Norway. Electronic address: havard.kauserud@ibv.uio.no.
Jazyk: angličtina
Zdroj: Fungal biology [Fungal Biol] 2015 Oct; Vol. 119 (10), pp. 940-945. Date of Electronic Publication: 2015 Jul 26.
DOI: 10.1016/j.funbio.2015.07.006
Abstrakt: In the fungal kingdom there is a high prevalence of morphologically defined species that includes closely related 'cryptic' biological species with similar phenotypes. Due to evolutionary processes like incomplete lineage sorting and introgression through hybridization, several independent DNA markers are essential to resolve closely related fungal species. In this study we wanted to analyze how many independent loci are necessary to reveal the cryptic species, using the genus Serpula as a model system. DNA sequences from ten different DNA loci, eight nuclear and two mitochondrial DNA markers, were obtained from various cryptic species within Serpula. The inclusion of five loci gave a highly confident separation of the cryptic species. Several other loci performed better than the standard DNA barcoding marker ITS in separating the cryptic species. The DNA loci tub, hsp, rpb2 and tef gave, on average, best support for the different cryptic species in single gene trees. We conclude that the analyses of a few but informative independent DNA loci, such as tub, hsp, rpb2 and tef in addition to the standard DNA barcode ITS, may give a good indication about the existence of cryptic species in fungi.
(Copyright © 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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