Development and characterization of reverse genetics system for the Indian West Nile virus lineage 1 strain 68856.

Autor: Pavitrakar DV; Encephalitis Group, National Institute of Virology, Microbial Containment Complex, Pashan, Pune 411021, India. Electronic address: dayapavitrakar@gmail.com., Ayachit VM; Encephalitis Group, National Institute of Virology, Microbial Containment Complex, Pashan, Pune 411021, India. Electronic address: vmayachit@gmail.com., Mundhra S; Encephalitis Group, National Institute of Virology, Microbial Containment Complex, Pashan, Pune 411021, India. Electronic address: sonal.mundhra@gmail.com., Bondre VP; Encephalitis Group, National Institute of Virology, Microbial Containment Complex, Pashan, Pune 411021, India. Electronic address: vpbondre@gmail.com.
Jazyk: angličtina
Zdroj: Journal of virological methods [J Virol Methods] 2015 Dec 15; Vol. 226, pp. 31-9. Date of Electronic Publication: 2015 Sep 24.
DOI: 10.1016/j.jviromet.2015.09.008
Abstrakt: Our previous studies on West Nile virus (WNV) strains isolated from human patients in India suggested substantial variation at the genetic level reflecting their variable pathogenesis. This study describes the development of reverse genetics system for a neurovirulent WNV isolate 68856 and its characterization. Full length viral cDNA was cloned into bacterial artificial chromosome (BAC) under the transcription control of T7 promoter. The RNA transcripts obtained by in vitro transcription were infectious in mammalian cells upon transfection. Cytopathic effect caused by synthetic RNA transcripts in mammalian cells, detection of cell associated viral protein after transfection and recovery of genetic markers in the progeny virus genome marked the successful development of reverse genetics system for WNV. Replication potential and plaque morphology of newly expressed virus along with its antigenic cross reactivity with the parental virus suggests synthesis of biologically identical replicative virus. Comparative neuropathogenesis studies in murine model indicated that the three genetic changes occurred in the recombinant virus during in vitro transcription has no impact on viral pathogenesis. The stable infectious cDNA clone generated from the neurovirulent Indian WNV strain will serve as a valuable experimental tool to study the viral factors contributing towards pathogenesis, host-virus interaction and immune evasion.
(Copyright © 2015. Published by Elsevier B.V.)
Databáze: MEDLINE
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