Significance of serum Il-9 levels in inflammatory bowel disease.

Autor:	Defendenti C; Laboratory Unit, Fatebenefratelli Hospital, Milan, Italy caterina.defendenti@fbf.milano.it., Sarzi-Puttini P; Rheumatology Unit, L. Sacco University Hospital, Milan, Italy., Saibeni S; Division of Pathology and Gastroenterology, Guido Salvini Hospital, Rho (Milan), Italy., Bollani S; Divisions of Pathology and Gastroenterology, Fatebenefratelli Hospital, Milan, Italy., Bruno S; Divisions of Pathology and Gastroenterology, Fatebenefratelli Hospital, Milan, Italy., Almasio PL; GI & Liver Unit, DI.BI.M.I.S. University of Palermo Policlinico, Palermo, Italy., Declich P; Laboratory Unit, Fatebenefratelli Hospital, Milan, Italy., Atzeni F; IRCCS Galeazzi Orthopedic Institute, Milan, Italy.
Jazyk:	angličtina
Zdroj:	International journal of immunopathology and pharmacology [Int J Immunopathol Pharmacol] 2015 Dec; Vol. 28 (4), pp. 569-75. Date of Electronic Publication: 2015 Sep 16.
DOI:	10.1177/0394632015600535
Abstrakt:	IL-9, which may be an inflammatory or regulatory cytokine, can be experimentally produced in a Th17 or modified Th2 context in the presence of T cell receptor (TCR) stimulation. The primary aim of this study was to measure serum IL-9 levels in patients with inflammatory bowel disease (IBD), and evaluate their relationships with the patients' clinical characteristics. The secondary aim was to determine the levels of interferon-γ (IFN (interferon)-γ), Th2 cytokines (IL-4, IL-5 and IL-13), and IL-6 in order to clarify the context of detectable peripheral cytokines in which IL-9 is produced.Venous blood samples of 43 IBD patients (20 with Crohn's disease [CD] and 23 with ulcerative colitis [UC]) were analysed by means of quantitative enzyme-linked immunosorbent assays using purified anti-human IL-4, IL-5, IL-13, IFN-γ, IL-9 and IL-6 antibodies, and the laboratory findings were statistically correlated with their clinical expression.None of the patients showed the peripheral presence of IL-4, IL-5 and IL-13. Forty (93%) were positive for IFN-γ, thus confirming the presence of Th1 in both UC and CD, and IFN-γ levels correlated with disease activity (P = 0.045). Eighteen patients (41%) were positive for IL-9, which was associated with a severe prognosis (P <0.001), and 72.2% of the IL-9-positive patients were also IL-6 positive. There was a significant correlation between disease severity and IL-9 in the CD patients (P <0.001), but not in the UC patients (P = 0.1).Our findings confirm the presence of common Th1 cytokines in UC and CD. However the IL-9 positivity indicates the presence of an alternative population of T cells that respond to antigen stimulation and condition the prognosis of IBD. The fact that the same serum IL-9 levels were differentially associated with clinical measures of CD and UC activity suggest that the same cytokine can be produced in different contexts. (© The Author(s) 2015.)
Databáze:	MEDLINE
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