Abundant toxin-related genes in the genomes of beneficial symbionts from deep-sea hydrothermal vent mussels.

Autor: Sayavedra L; Max Planck Institute for Marine Microbiology, Bremen, Germany., Kleiner M; Max Planck Institute for Marine Microbiology, Bremen, Germany., Ponnudurai R; Institute of Pharmacy, Ernst-Moritz-Arndt-University, Greifswald, Germany., Wetzel S; Max Planck Institute for Marine Microbiology, Bremen, Germany., Pelletier E; Genoscope - Centre National de Séquençage, Commissariat à l'énergie atomique et aux énergies alternatives, Evry, France.; Metabolic Genomics Group, Commissariat à l'énergie atomique et aux énergies alternatives, Evry, France.; University of Évry-Val d'Essonne, Evry, France., Barbe V; Genoscope - Centre National de Séquençage, Commissariat à l'énergie atomique et aux énergies alternatives, Evry, France., Satoh N; Marine Genomics Unit, Okinawa Institute of Science and Technology, Onna, Japan., Shoguchi E; Marine Genomics Unit, Okinawa Institute of Science and Technology, Onna, Japan., Fink D; Max Planck Institute for Marine Microbiology, Bremen, Germany., Breusing C; Evolutionary Ecology, GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany., Reusch TB; Evolutionary Ecology, GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany., Rosenstiel P; Institute of Clinical Molecular Biology, Kiel, Germany., Schilhabel MB; Institute of Clinical Molecular Biology, Kiel, Germany., Becher D; Institute of Marine Biotechnology, Greifswald, Germany.; Institute of Microbiology, Ernst-Moritz-Arndt-University, Greifswald, Germany., Schweder T; Institute of Pharmacy, Ernst-Moritz-Arndt-University, Greifswald, Germany.; Institute of Marine Biotechnology, Greifswald, Germany., Markert S; Institute of Pharmacy, Ernst-Moritz-Arndt-University, Greifswald, Germany.; Institute of Marine Biotechnology, Greifswald, Germany., Dubilier N; Max Planck Institute for Marine Microbiology, Bremen, Germany.; University of Bremen, Bremen, Germany., Petersen JM; Max Planck Institute for Marine Microbiology, Bremen, Germany.
Jazyk: angličtina
Zdroj: ELife [Elife] 2015 Sep 15; Vol. 4, pp. e07966. Date of Electronic Publication: 2015 Sep 15.
DOI: 10.7554/eLife.07966
Abstrakt: Bathymodiolus mussels live in symbiosis with intracellular sulfur-oxidizing (SOX) bacteria that provide them with nutrition. We sequenced the SOX symbiont genomes from two Bathymodiolus species. Comparison of these symbiont genomes with those of their closest relatives revealed that the symbionts have undergone genome rearrangements, and up to 35% of their genes may have been acquired by horizontal gene transfer. Many of the genes specific to the symbionts were homologs of virulence genes. We discovered an abundant and diverse array of genes similar to insecticidal toxins of nematode and aphid symbionts, and toxins of pathogens such as Yersinia and Vibrio. Transcriptomics and proteomics revealed that the SOX symbionts express the toxin-related genes (TRGs) in their hosts. We hypothesize that the symbionts use these TRGs in beneficial interactions with their host, including protection against parasites. This would explain why a mutualistic symbiont would contain such a remarkable 'arsenal' of TRGs.
Databáze: MEDLINE