Conditional deletion of nonmuscle myosin II-A in mouse tongue epithelium results in squamous cell carcinoma.
| Autor: | Conti MA; Laboratory of Molecular Cardiology, NHLBI, Bethesda, MD 20814, USA., Saleh AD; Head and Neck Surgery Branch, NIDCD, Bethesda, MD 20892, USA., Brinster LR; Division of Veterinary Resources, Office of Research Services, NIH, Bethesda, Maryland 20892-5520, USA., Cheng H; Head and Neck Surgery Branch, NIDCD, Bethesda, MD 20892, USA., Chen Z; Head and Neck Surgery Branch, NIDCD, Bethesda, MD 20892, USA., Cornelius S; Head and Neck Surgery Branch, NIDCD, Bethesda, MD 20892, USA., Liu C; Transgenic Core Facility, NHLBI, NIH, Bethesda, MD, 20892, USA., Ma X; Laboratory of Molecular Cardiology, NHLBI, Bethesda, MD 20814, USA., Van Waes C; Head and Neck Surgery Branch, NIDCD, Bethesda, MD 20892, USA., Adelstein RS; Laboratory of Molecular Cardiology, NHLBI, Bethesda, MD 20814, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Scientific reports [Sci Rep] 2015 Sep 15; Vol. 5, pp. 14068. Date of Electronic Publication: 2015 Sep 15. |
| DOI: | 10.1038/srep14068 |
| Abstrakt: | To investigate the contribution of nonmuscle myosin II-A (NM II-A) to early cardiac development we crossed Myh9 floxed mice and Nkx2.5 cre-recombinase mice. Nkx2.5 is expressed in the early heart (E7.5) and later in the tongue epithelium. Mice homozygous for deletion of NM II-A (A(Nkx)/A(Nkx)) are born at the expected ratio with normal hearts, but consistently develop an invasive squamous cell carcinoma (SCC) of the tongue (32/32 A(Nkx)/A(Nkx)) as early as E17.5. To assess reproducibility a second, independent line of Myh9 floxed mice derived from a different embryonic stem cell clone was tested. This second line also develops SCC indistinguishable from the first (15/15). In A(Nkx)/A(Nkx) mouse tongue epithelium, genetic deletion of NM II-A does not affect stabilization of TP53, unlike a previous report for SCC. We attribute the consistent, early formation of SCC with high penetrance to the role of NM II in maintaining mitotic stability during karyokinesis. |
| Databáze: | MEDLINE |
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